Human CEACAM-3/CD66d Biotinylated Antibody

Catalog #: BAF4166 Datasheet / COA / SDS

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BAF4166

Detection of CEACAM‑3/CD66d in Human Granulocytes by Flow Cytometry.

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Human CEACAM-3/CD66d Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEACAM‑3/CD66d in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human (rh) CEACAM-1 is observed, 10% cross-reactivity with rhCEACAM-6 is observed, and 5% cross-reactivity with rhCEACAM-4 and rhCEACAM‑5 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CEACAM‑3/CD66d isoform 1
Lys35-Gly155
Accession # P40198

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CEACAM‑3/CD66d

Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CEACAM‑3/CD66d in Human Granulocytes by Flow Cytometry. Human whole blood granulocytes were stained with Sheep Anti-Human CEACAM-3/ CD66d Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4166, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-3/CD66d

CEACAM-3 (carcinoembryonic antigen-related cell adhesion molecule 3; also CD66d and CGM1) is a 31‑34 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and serves as a microbial receptor for a wide range of microorganisms. Mature human CEACAM-3 is a 218 amino acid (aa) type I transmembrane glycoprotein. Its extracellular domain (aa 35‑155) shows one V-type Ig-like domain (aa 35‑142). There are two alternate splice forms. One shows a 40 aa substitution for the C-terminal 116 amino acids (aa 137‑252), while a second shows a 31 aa substitution for the C-terminal 71 amino acids. No definitive rodent CEACAM-3 has been reported.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 3

Entrez Gene IDs

1084 (Human); 384557 (Mouse); 24926 (Rat)

Alternate Names

CD66d; CEA; CEACAM3 carcinoembryonic antigen-related cell adhesion molecule 3; CEACAM-3; CGM1; W264; W282