Human CD8 alpha Fluorescein-conjugated Antibody

Catalog #: FAB1509F
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB1509F-100
FAB1509F-025

Detection of CD8 alpha in Human Blood Lymphocytes by Flow Cytometry.

1 Image

All CD8 Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD8 alpha Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD8 alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with mouse CD8 alpha is observed.

Source

Monoclonal Mouse IgG_2B Clone # 37006

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD8 alpha
Ser22-Val198
Accession # NP_741969

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CD8 alpha in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD8a Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1509F) or (B) Mouse IgG2B isotype control antibody (Catalog # IC0041F) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD8

CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Alternate Names

CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CD8 alpha Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

Streamlined selection of cancer antigens for vaccine development through integrative multi-omics and high-content cell imaging
Authors: KC Han, D Park, S Ju, YE Lee, SH Heo, YA Kim, JE Lee, Y Lee, KH Park, SH Park, HJ Lee, C Lee, M Jang
Sci Rep, 2020-04-03;10(1):5885.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Potential role for IL-7 in Fas-mediated T cell apoptosis during HIV infection.
Authors: Fluur C, De Milito A, Fry TJ, Vivar N, Eidsmo L, Atlas A, Federici C, Matarrese P, Logozzi M, Rajnavolgyi E, Mackall CL, Fais S, Chiodi F, Rethi B
J. Immunol., 2007-04-15;178(8):5340-50.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry