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Human CD3 epsilon APC-conjugated Antibody

Catalog #: FAB100A
5 Citations Datasheet / COA / SDS
Clone UCHT1 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB100A-100
FAB100A-025
Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD3 epsilon APC-conjugated Antibody Summary

Specificity
Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
Source
Monoclonal Mouse IgG1 Clone # UCHT1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human thymocytes (1)
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD3e antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD3 epsilon

CD3 epsilon is a key signaling molecule that participates in the formation of the T Cell Receptor (TCR). The alpha beta TCR is composed of six distinct subunits. There is an alpha -beta disulfide-linked heterodimer that recognizes antigen, and this is accompanied by four CD3 subunits that transduce an intracellular signal. CD3 epsilon forms a heterodimer with both CD3 gamma and CD3δ, creating two signaling complexes. These two heterodimers are supplemented by a CD3 zeta zeta homodimer that lies in the center of the TCR complex, generating a fully-functional TCR (2). 

References
  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
  2. Call, M.E. and K.W. Wucherpfennig (2005) Annu. Rev. Immunol. 23: 101
Entrez Gene IDs
916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)
Alternate Names
CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

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Citations for Human CD3 epsilon APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Effective Cytotoxic T Lymphocyte Targeting of Persistent HIV-1 during Antiretroviral Therapy Requires Priming of Naive CD8+ T Cells
    Authors: Kellie N Smith
    MBio, 2016-05-31;7(3):.
  2. Comparative Multi-Donor Study of IFNgamma Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays.
    Authors: Hagen J, Zimmerman R, Goetz C, Bonnevier J, Houchins J, Reagan K, Kalyuzhny A
    Cells, 2015-02-11;4(1):84-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. HIV-1 infection is characterized by profound depletion of CD161+ Th17 cells and gradual decline in regulatory T cells.
    Authors: Prendergast A, Prado JG, Kang YH, Chen F, Riddell LA, Luzzi G, Goulder P, Klenerman P
    AIDS, 2010-02-20;24(4):491-502.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Exogenous TERC alone can enhance proliferative potential, telomerase activity and telomere length in lymphocytes from dyskeratosis congenita patients.
    Authors: Kirwan M, Beswick R, Vulliamy T, Nathwani AC, Walne AJ, Casimir C, Dokal I
    Br. J. Haematol., 2008-11-20;144(5):771-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis.
    Authors: Garcia G, Humbert M, Capel F, Rimaniol AC, Escourrou P, Emilie D, Godot V
    Allergy, 2007-02-01;62(2):170-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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