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Human CD14 PE-conjugated Antibody

Catalog #: FAB3832P
14 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3832P-100
FAB3832P-025
Detection of CD14 in Human PBMCs by Flow Cytometry.
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Citations (14)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD14 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD14 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 134620
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD14
Thr20-Cys352
Accession # P08571
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD14 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD14 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

References
  1. Wright, S.D. et al. (1990) Science 249:1431.
  2. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
  3. Beutler, B. (2000) Current Opinion in Immunology 12:20.
  4. Stelter, F. (2000) Chem. Immunol. 74:25.
Entrez Gene IDs
929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
Alternate Names
CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

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Citations for Human CD14 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Relation of Neutrophil Gelatinase-Associated Lipocalin Overexpression to the Resistance to Apoptosis of Tumor B Cells in Chronic Lymphocytic Leukemia
    Authors: B Bauvois, E Pramil, L Jondrevill, E Chapiro, C Quiney, K Maloum, SA Susin, F Nguyen-Kha
    Cancers (Basel), 2020-07-31;12(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial
    Authors: S Sbrana, J Campolo, A Clemente, L Bastiani, A Cecchettin, E Ceccherini, C Caselli, D Neglia, O Parodi, D Chiappino, JM Smit, AJ Scholte, G Pelosi, S Rocchiccio
    Biomed Res Int, 2020-07-27;2020(0):8748934.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. TGF beta Is Specifically Upregulated on Circulating CD14++ CD16+ and CD14+ CD16++ Monocytes in Patients with Atrial Fibrillation and Severe Atrial Fibrosis
    Authors: David Heinzmann, Stefan Fu beta, Saskia v. Ungern-Sternberg, Jürgen Schreieck, Meinrad Gawaz, Michael Gramlich et al.
    Cellular Physiology and Biochemistry
  4. Signaling Lymphocyte Activation Molecule Family 5 Enhances Autophagy and Fine-Tunes Cytokine Response in Monocyte-Derived Dendritic CellsStabilization of Interferon Regulatory Factor 8
    Authors: Z Agod, K Pazmandi, D Bencze, G Vereb, T Biro, A Szabo, E Rajnavolgy, A Bacsi, P Engel, A Lanyi
    Front Immunol, 2018-01-26;9(0):62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Study of the microRNA expression profile of foreskin derived mesenchymal stromal cells following inflammation priming
    Authors: H Fayyad-Kaz, M Fayyad-Kaz, B Badran, D Bron, L Lagneaux, M Najar
    J Transl Med, 2017-01-13;15(1):10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. TLR4/CD14 Variants-Related Serologic and Immunologic Dys-Regulations Predict Severe Sepsis in Febrile De-Compensated Cirrhotic Patients
    PLoS ONE, 2016-11-18;11(11):e0166458.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. The Macrophage Inhibitor CNI-1493 Blocks Metastasis in a Mouse Model of Ewing Sarcoma through Inhibition of Extravasation
    Authors: Anthony J. Hesketh, Caroline Maloney, Christopher A. Behr, Morris C. Edelman, Richard D. Glick, Yousef Al-Abed et al.
    PLOS ONE
  8. Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues
    Authors: ZHENXIN WANG, JIANFENG YANG, YANBO ZHU, YUN ZHU, BIN ZHANG, YINGHUI ZHOU
    Oncology Letters
  9. Physical Exercise Reduces the Expression of RANTES and Its CCR5 Receptor in the Adipose Tissue of Obese Humans
    Authors: Engin Baturcam, Jehad Abubaker, Ali Tiss, Mohamed Abu-Farha, Abdelkrim Khadir, Fahad Al-Ghimlas et al.
    Mediators of Inflammation
  10. CXCR4 positive and angiogenic monocytes in myocardial infarction.
    Thromb Haemost, 2012-12-06;109(2):255-62.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  11. Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
    Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
    PLoS ONE, 2012-10-09;7(10):e47187.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Human mesenchymal stromal cells express CD14 cross-reactive epitopes.
    Authors: Pilz GA, Braun J, Ulrich C, Felka T, Warstat K, Ruh M, Schewe B, Abele H, Larbi A, Aicher WK
    Cytometry A, 2011-07-06;79(8):635-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Epitope specificity and clonality of EBV-specific CTLs used to treat posttransplant lymphoproliferative disease.
    Authors: McAulay KA, Haque T, Urquhart G, Bellamy C, Guiretti D, Crawford DH
    J. Immunol., 2009-03-15;182(6):3892-901.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Herpes simplex virus-1 up-regulates IL-15 gene expression in monocytic cells through the activation of protein tyrosine kinase and PKC zeta/lambda signaling pathways.
    Authors: Ahmad R, Ennaciri J, Cordeiro P, El Bassam S, Menezes J
    J. Mol. Biol., 2006-12-28;367(1):25-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human CD14 PE-conjugated Antibody
By Anonymous on 02/26/2021
Application: Flow Sample Tested: Peripheral blood B cells Species: Human
Flow Cytometry Human CD14 PE-conjugated Antibody FAB3832P