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Human CCR2 Antibody

Catalog #: MAB150
45 Citations 1 Review Datasheet / COA / SDS
Clone 48607 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB150-100
MAB150-SP
Detection of CCR2 in Human Blood Monocytes by Flow Cytometry.
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Product Details
Citations (45)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR-2 transfectants but not the parental cell line or CCR-5 transfectants.
Source
Monoclonal Mouse IgG2B Clone # 48607
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human CCR2
Met1-Leu360
Accession # NP_001116868
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-50 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR2 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CCR2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCR2 Monoclonal Antibody (Catalog # MAB150) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry CCR2 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

CCR2 in Human Tonsil. CCR2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CCR2 Monoclonal Antibody (Catalog # MAB150) at 50 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR2

CCR2 is a G-protein linked seven transmembrane domain spanning chemokine receptor that preferentially binds monocyte chemoattractant proteins-1 and -3 (MCP-1 and MCP-3). Two isoforms of this receptor (CCR2A and CCR2B) are expressed on cell surfaces as a result of alternate splicing from the same gene. These two CCR2 variants differ only at their intracellular carboxyl terminals, with the CCR2A form possessing 14 additional amino acids. This may provide a mechanism by which cells responding to similar extracellular ligands can activate different intracellular second messengers. Cells that respond to the action of MCP-1 and therefore are likely to express CCR2 receptors, include monocytes, T cells, NK cells, basophils, mast cells and dendritic cells. A recent report suggests that B cells may also express CCR2 receptors. The recognition that a variety of chemokine receptors, including CCR2, can serve as HIV fusion co-factors and as facilitators of T cell recruitment during inflammation makes chemokine receptor monitoring an important exercise in elucidating the HIV infection process and the regulation of inflammatory reactions.

Entrez Gene IDs
729230 (Human); 12772 (Mouse); 60463 (Rat)
Alternate Names
C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-R; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

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Citations for Human CCR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

45 Citations: Showing 1 - 10
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  1. alpha1B/D-adrenoceptors regulate chemokine receptor-mediated leukocyte migration via formation of heteromeric receptor complexes
    Authors: GA Enten, X Gao, HR Strzelinsk, M Weche, SB Liggett, M Majetschak
    Proceedings of the National Academy of Sciences of the United States of America, 2022-05-10;119(20):e2123511119.
  2. Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets.
    Authors: Cassetta L, Fragkogianni S, Sims AH et al.
    Cancer Cell.
  3. CCL2/CCR2 Expression in Locally Advanced Prostate Cancer and Patient Long-Term Outcome: 10-Year Results from the TROG 03.04 RADAR Trial
    Authors: Marsland, M;Jiang, CC;Faulkner, S;Steigler, A;McEwan, K;Jobling, P;Oldmeadow, C;Delahunt, B;Denham, JW;Hondermarck, H;
    Cancers
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  4. Parkinson's disease-derived ?-synuclein assemblies combined with chronic-type inflammatory cues promote a neurotoxic microglial phenotype
    Authors: Yildirim-Balatan, C;Fenyi, A;Besnault, P;Gomez, L;Sepulveda-Diaz, JE;Michel, PP;Melki, R;Hunot, S;
    Journal of neuroinflammation
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry/Immunofluorescence
  5. Innate Immune Signaling in Hearts and Buccal Mucosa Cells of Patients with Arrhythmogenic Cardiomyopathy
    Authors: Bueto-Beni, C;Tafuni, A;Chelko, SP;Sheppard, MN;Field, E;Tollit, J;Heenan, IK;Barnes, A;Taylor, MR;Mestroni, L;Kaski, JP;Saffitz, JE;Asimaki, A;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Dynamic bioinspired coculture model for probing ER+ breast cancer dormancy in the bone marrow niche
    Authors: L Pradhan, D Moore, EM Ovadia, SL Swedzinski, T Cossette, RA Sikes, K van Golen, AM Kloxin
    Science Advances, 2023-03-08;9(10):eade3186.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines
    Authors: Shengli Song, Miriam Manook, Jean Kwun, Annette M. Jackson, Stuart J. Knechtle, Garnett Kelsoe
    Communications Biology
  8. Atlas of clinically distinct cell states and ecosystems across human solid tumors
    Authors: Bogdan A. Luca, Chloé B. Steen, Magdalena Matusiak, Armon Azizi, Sushama Varma, Chunfang Zhu et al.
    Cell
  9. Expression of CCL2/CCR2 signaling proteins in breast carcinoma cells is associated with invasive progression
    Authors: WB Fang, D Sofia Acev, C Smart, B Zinda, N Alissa, K Warren, G Fraga, LC Huang, Y Shyr, W Li, L Xie, V Staggs, Y Hong, F Behbod, N Cheng
    Scientific Reports, 2021-04-22;11(1):8708.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  10. Development and application of a high-content virion display human GPCR array
    Authors: Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al.
    Nature Communications
  11. Regulation of C-C chemokine receptor 5 (CCR5) stability by Lys-197 and by transmembrane protein aptamers that target it for lysosomal degradation
    Authors: LM Petti, SA Marlatt, Y Luo, EH Scheideman, A Shelar, D DiMaio
    J. Biol. Chem., 2018-04-20;0(0):.
    Applications: Flow Cytometry
  12. Regulatory T-cells from pancreatic lymphnodes of patients with type-1 diabetes express increased levels of microRNA miR-125a-5p that limits CCR2 expression
    Authors: G Sebastiani, G Ventriglia, A Stabilini, C Socci, C Morsiani, A Laurenzi, L Nigi, C Formichi, B Mfarrej, A Petrelli, G Fousteri, TM Brusko, F Dotta, M Battaglia
    Sci Rep, 2017-07-31;7(1):6897.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Macrophage infiltration and genetic landscape of undifferentiated uterine sarcomas
    Authors: J Przybyl, M Kowalewska, A Quattrone, B Dewaele, V Vanspauwen, S Varma, S Vennam, AM Newman, M Swierniak, E Baku?a-Zal, JA Siedlecki, M Bidzinski, J Cools, M van de Rij, M Debiec-Ryc
    JCI Insight, 2017-06-02;2(11):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. CSF-1 regulates the function of monocytes in Crohn's disease patients in remission
    Authors: JC Nieto, C Zamora, E Cantó, E Garcia-Pla, J Gordillo, MA Ortiz, C Juárez, S Vidal
    Sci Rep, 2017-03-07;7(1):92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity
    Authors: Zaidoon Al-Jaderi
    Front Immunol, 2016-10-19;7(0):437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Evidence for chemokine synergy during neutrophil migration in ARDS
    Thorax, 2016-08-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals.
    Authors: Williams D, Anastos K, Morgello S, Berman J
    J Leukoc Biol, 2014-11-24;97(2):401-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. HIV-1 Nef down-modulates C-C and C-X-C chemokine receptors via ubiquitin and ubiquitin-independent mechanism.
    Authors: Chandrasekaran, Prabha, Moore, Victoria, Buckley, Monica, Spurrier, Joshua, Kehrl, John H, Venkatesan, Sundarar
    PLoS ONE, 2014-01-29;9(1):e86998.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. PC3-secreted microprotein is a novel chemoattractant protein and functions as a high-affinity ligand for CC chemokine receptor 2.
    Authors: Pei X, Sun Q, Zhang Y, Wang P, Peng X, Guo C, Xu E, Zheng Y, Mo X, Ma J, Chen D, Zhang Y, Zhang Y, Song Q, Guo S, Shi T, Zhang Z, Ma D, Wang Y
    J Immunol, 2014-01-17;192(4):1878-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Changes of peripheral TGF-beta1 depend on monocytes-derived macrophages in Huntington disease.
    Authors: Di Pardo A, Alberti S, Maglione V, Amico E, Cortes E, Elifani F, Battaglia G, Busceti C, Nicoletti F, Vonsattel J, Squitieri F
    Mol Brain, 2013-12-13;6(0):55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Derivation of injury-responsive dendritic cells for acute brain targeting and therapeutic protein delivery in the stroke-injured rat.
    Authors: Manley, Nathan C, Caso, Javier R, Works, Melissa, Cutler, Andrew B, Zemlyak, Ilona, Sun, Guohua, Munhoz, Carolina, Chang, Sydney, Sorrells, Shawn F, Ermini, Florian, Decker, Johannes, Bertrand, Anthony, Dinkel, Klaus M, Steinberg, Gary K, Sapolsky, Robert M
    PLoS ONE, 2013-04-16;8(4):e61789.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Absence of CC chemokine receptors 2a and 2b from human adipose lineage cells.
    Authors: Koenig C, Fischer-Posovszky P, Rojewski M, Tews D, Schrezenmeier H, Wabitsch M, Gierschik P, Moepps B
    Mol Cell Endocrinol, 2013-01-29;369(1):72-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Functional diversity of human vaginal APC subsets in directing T-cell responses.
    Authors: Duluc, D, Gannevat, J, Anguiano, E, Zurawski, S, Carley, M, Boreham, M, Stecher, J, Dullaers, M, Banchereau, J, Oh, S
    Mucosal Immunol, 2012-11-07;6(3):626-38.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  24. In vitro migration capacity of human adipose tissue-derived mesenchymal stem cells reflects their expression of receptors for chemokines and growth factors
    Authors: Sun Jin Baek, Sung Keun Kang, Jeong Chan Ra
    Experimental and Molecular Medicine
  25. Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
    Authors: Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
    Int. J. Cancer, 2010-07-15;127(2):332-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Polarization of Migrating Monocytic Cells Is Independent of PI 3-Kinase Activity
    Authors: Silvia Volpe, Sylvia Thelen, Thomas Pertel, Martin J. Lohse, Marcus Thelen
    PLoS ONE
  27. Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells.
    Authors: Dagouassat M, Suffee N, Hlawaty H, Haddad O, Charni F, Laguillier C, Vassy R, Martin L, Schischmanoff PO, Gattegno L, Oudar O, Sutton A, Charnaux N
    Int. J. Cancer, 2010-03-01;126(5):1095-108.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Comparison of gene expression profiles between human and mouse monocyte subsets.
    Authors: Ingersoll MA, Spanbroek R, Lottaz C
    Blood, 2009-11-12;115(3):e10-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6.
    Authors: Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ
    Int. Immunol., 2009-07-10;21(8):991-1001.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. Chemokine-enhanced chemotaxis of lymphangioleiomyomatosis cells with mutations in the tumor suppressor TSC2 gene.
    Authors: Pacheco-Rodriguez G, Kumaki F, Steagall WK, Zhang Y, Ikeda Y, Lin JP, Billings EM, Moss J
    J. Immunol., 2009-02-01;182(3):1270-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  31. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008-12-01;181(11):8053-67.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC-P, Neutralization
  32. Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
    Authors: Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA
    Am. J. Transplant., 2008-07-01;8(7):1512-22.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  33. Synergy between coproduced CC and CXC chemokines in monocyte chemotaxis through receptor-mediated events.
    Authors: Gouwy M, Struyf S, Noppen S, Schutyser E, Springael JY, Parmentier M, Proost P, Van Damme J
    Mol. Pharmacol., 2008-05-09;74(2):485-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system affects cell motility in cultured human podocytes.
    Authors: Burt D, Salvidio G, Tarabra E, Barutta F, Pinach S, Dentelli P, Camussi G, Perin PC, Gruden G
    Am. J. Pathol., 2007-11-30;171(6):1789-99.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  35. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
    Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
    Stem Cells, 2007-03-29;25(7):1737-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  36. HIV-1 tat protein induces a migratory phenotype in human fetal microglia by a CCL2 (MCP-1)-dependent mechanism: possible role in NeuroAIDS.
    Authors: Eugenin EA, Dyer G, Calderon TM, Berman JW
    Glia, 2005-03-01;49(4):501-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  37. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004-12-01;173(11):6592-602.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  38. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004-12-01;114(11):1640-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  39. Distinct transcriptional programs activated by interleukin-10 with or without lipopolysaccharide in dendritic cells: induction of the B cell-activating chemokine, CXC chemokine ligand 13.
    Authors: Perrier P, Martinez FO, Locati M, Bianchi G, Nebuloni M, Vago G, Bazzoni F, Sozzani S, Allavena P, Mantovani A
    J. Immunol., 2004-06-01;172(11):7031-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  40. Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.
    Authors: Metelitsa LS, Wu HW, Wang H, Yang Y, Warsi Z, Asgharzadeh S, Groshen S, Wilson SB, Seeger RC
    J. Exp. Med., 2004-05-03;199(9):1213-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  41. A CCR2-V64I polymorphism affects stability of CCR2A isoform.
    Authors: Nakayama EE, Tanaka Y, Nagai Y, Iwamoto A, Shioda T
    AIDS, 2004-03-26;18(5):729-38.
    Species: Human, Primate - Cholrocebus pygerythrus (Vervet Monkey)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  42. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003-12-04;103(7):2806-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  43. Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
    Authors: Campbell J, Qin S, Unutmaz D, Soler D, Murphy K, Hodge M, Wu L, Butcher E
    J Immunol, 2001-06-01;166(11):6477-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  44. An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer
    Authors: Johnson BE, Creason AL, Stommel JM et al.
    Cell reports. Medicine
  45. Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function.
    Authors: Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW
    J. Biol. Chem., 1999-04-02;274(14):9617-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human CCR2 Antibody
By Anonymous on 08/18/2021
Application: IHC Sample Tested: Lung tissue Species: Human
Immunohistochemistry Human CCR2 Antibody MAB150