Home / CCL11/Eotaxin / Human CCL11/Eotaxin DuoSet ELISA

Human CCL11/Eotaxin DuoSet ELISA

Catalog #: DY320
17 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY320
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human CCL11 / Eotaxin ELISA Standard Curve
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Product Details
Procedure
Citations (17)
FAQs
Supplemental Products
Reviews (2)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human CCL11/Eotaxin DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CCL11/Eotaxin. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human CCL11 / Eotaxin ELISA Standard Curve View Larger

Human CCL11 / Eotaxin ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL11/Eotaxin

CCL11/Eotaxin is a chemokine that interacts with CCR3 to induce eosinophil, basophil, dendritic cell, and Th2 cell chemoattraction during inflammatory and allergic reactions. Its production is upregulated in airway epithelium, alveolar macrophages, eosinophils, smooth muscle cells, chondrocytes, and fibroblasts following allergen or inflammatory challenge. CCL11 is also involved in the growth of myeloid cell progenitors and the differentiation of mast cells.

Entrez Gene IDs:
6356 (Human); 20292 (Mouse)
Alternate Names:
C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human CCL11/Eotaxin DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Association between the sinus microbiota with eosinophilic inflammation and prognosis in chronic rhinosinusitis with nasal polyps
    Authors: JH Kim, SH Kim, JY Lim, D Kim, IS Jeong, DK Lee, YJ Jang
    Exp. Mol. Med., 2020-06-29;52(6):978-987.
    Species: Human
    Sample Types: Tissue Homogenates
  2. Berberine Inhibits Pro-inflammatory Cytokine-induced IL-6 and CCL11 Production via Modulation of STAT6 Pathway in Human Bronchial Epithelial Cells
    Authors: J Ma, CC Chan, WC Huang, ML Kuo
    Int J Med Sci, 2020-06-08;17(10):1464-1473.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. A Cross-Sectional Study Evaluating the Effects of Resistance Exercise on Inflammation and Neurotrophic Factors in Elderly Women with Obesity
    Authors: HT Roh, SY Cho, WY So
    J Clin Med, 2020-03-20;9(3):.
    Species: Human
    Sample Types: Serum
  4. Telomere Length, Oxidative Stress, Inflammation and BDNF Levels in Siblings of Patients with Bipolar Disorder: Implications for Accelerated Cellular Aging
    Authors: MP Vasconcelo, GR Fries, C Gubert, BT Dos Santos, A Fijtman, J Sartori, P Ferrari, LK Grun, MM Parisi, FT Guma, FM Barbé-Tuan, F Kapczinski, AR Rosa, LN Yatham, M Kauer-Sant
    Int. J. Neuropsychopharmacol., 2017-06-01;0(0):.
    Species: Human
    Sample Types: Serum
  5. The MNK-1/eIF4E pathway as a new therapeutic pathway to target inflammation and remodelling in asthma
    Cell Signal, 2016-07-11;28(10):1555-1562.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Regulation of IL-17A responses in human airway smooth muscle cells by Oncostatin M.
    Authors: Kwofie K, Scott M, Rodrigues R, Guerette J, Radford K, Nair P, Richards C
    Respir Res, 2015-02-07;16(0):14.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Pentraxin 3 (PTX3) expression in allergic asthmatic airways: role in airway smooth muscle migration and chemokine production.
    Authors: Zhang J, Shan L, Koussih L, Redhu NS, Halayko AJ, Chakir J, Gounni AS
    PLoS ONE, 2012-04-18;7(4):e34965.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Plasma levels of procalcitonin and eight additional inflammatory molecules in febrile neutropenic patients.
    Authors: Neuenschwander LC, Bittencourt H, Ribeiro AF, Teixeira AL, Teixeira MM, Teixeira JC, Nobre V
    Clinics (Sao Paulo), 2011-01-01;66(10):1699-705.
    Species: Human
    Sample Types: Plasma
  9. Genetic variants of CC chemokine genes in experimental autoimmune encephalomyelitis, multiple sclerosis and rheumatoid arthritis.
    Authors: Ockinger J, Stridh P, Beyeen AD, Lundmark F, Seddighzadeh M, Oturai A, Sorensen PS, Lorentzen AR, Celius EG, Leppa V, Koivisto K, Tienari PJ, Alfredsson L, Padyukov L, Hillert J, Kockum I, Jagodic M, Olsson T
    Genes Immun., 2009-10-29;11(2):142-54.
    Species: Human
    Sample Types: CSF
  10. Abnormalities in serum chemokine levels in euthymic patients with bipolar disorder.
    Authors: Brietzke E, Kauer-Sant'Anna M, Teixeira AL, Kapczinski F
    Brain Behav. Immun., 2009-05-04;23(8):1079-82.
    Species: Human
    Sample Types: Serum
  11. CD8 chemokine receptors in chronic obstructive pulmonary disease.
    Authors: Smyth LJ, Starkey C, Gordon FS, Vestbo J, Singh D
    Clin. Exp. Immunol., 2008-08-22;154(1):56-63.
    Species: Human
    Sample Types: BALF
  12. IL-13 involvement in eosinophilic esophagitis: transcriptome analysis and reversibility with glucocorticoids.
    Authors: Blanchard C, Mingler MK, Vicario M, Abonia JP, Wu YY, Lu TX, Collins MH, Putnam PE, Wells SI, Rothenberg ME
    J. Allergy Clin. Immunol., 2007-12-01;120(6):1292-300.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Increased serum levels of CCL11/eotaxin in schizophrenia.
    Authors: Teixeira AL, Reis HJ, Nicolato R, Brito-Melo G, Correa H, Teixeira MM, Romano-Silva MA
    Prog. Neuropsychopharmacol. Biol. Psychiatry, 2007-11-23;32(3):710-4.
    Species: Human
    Sample Types: Serum
  14. Preadipocyte response and impairment of differentiation in an inflammatory environment.
    Authors: Poulain-Godefroy O, Froguel P
    Biochem. Biophys. Res. Commun., 2007-03-19;356(3):662-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Toll-like receptor 2, 3, and 4 expression and function in human airway smooth muscle.
    Authors: Sukkar MB, Xie S, Khorasani NM, Kon OM, Stanbridge R, Issa R, Chung KF
    J. Allergy Clin. Immunol., 2006-07-03;118(3):641-8.
    Species: Human
    Sample Types: Whole Cells
  16. Influence of eotaxin 67G>A polymorphism on plasma eotaxin concentrations in myocardial infarction survivors and healthy controls.
    Authors: Sheikine Y, Olsen B, Gharizadeh B, Jatta K, Tornvall P, Ghaderi M
    Atherosclerosis, 2006-02-28;189(2):458-63.
    Species: Human
    Sample Types: Plasma
  17. Eotaxin-3 and a uniquely conserved gene-expression profile in eosinophilic esophagitis.
    Authors: Assa'ad AH
    J. Clin. Invest., 2006-02-01;116(2):536-47.
    Species: Human
    Sample Types: Tissue Homogenates

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Reviews for Human CCL11/Eotaxin DuoSet ELISA

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Human CCL11/Eotaxin DuoSet ELISA
By Anonymous on 06/09/2020
Sample Tested: Serum and Plasma

We used this kit to quantify the CCL11 in human serum and plasma samples. Works very well and generates a very good standard curve.

Human CCL11/Eotaxin DuoSet ELISA DY320
Human CCL11/Eotaxin DuoSet ELISA
By Balaji Mahender on 12/20/2017
Sample Tested: EDTA Plasma