Home / CCL11/Eotaxin / Human CCL11/Eotaxin Biotinylated Antibody

Human CCL11/Eotaxin Biotinylated Antibody

Catalog #: BAF320
8 Citations 1 Review Datasheet / COA / SDS
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BAF320
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL11/Eotaxin Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL11/Eotaxin in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant mouse (rm) CCL11, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rhMIP-1 alpha, and rhRANTES is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL11/Eotaxin (R&D Systems, Catalog # 320-EO)
Gly24-Pro97
Accession # Q6I9T4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL11/Eotaxin (Catalog # 320-EO)

Human CCL11/Eotaxin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CCL11/Eotaxin Antibody (Catalog # MAB320)

Standard: Recombinant Human CCL11/Eotaxin Protein (Catalog # 320-EO)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2 and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1 - 3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).

References
  1. Kitamura, M. et al. (1996) J. Biol. Chem 271:7725.
  2. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  3. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
  4. Choe, H. et al. (1996) Cell 85:1135.
 
Entrez Gene IDs
6356 (Human); 20292 (Mouse)
Alternate Names
C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

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Citations for Human CCL11/Eotaxin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. The Global Phosphorylation Landscape of SARS-CoV-2 Infection
    Authors: Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al.
    Cell
  2. Placental immune response to apple allergen in allergic mothers
    Authors: Martina Sandberg Abelius, Uta Enke, Frauke Varosi, Heike Hoyer, Ekkehard Schleussner, Maria C. Jenmalm et al.
    Journal of Reproductive Immunology
  3. A Th1/Th2-associated chemokine imbalance during infancy in children developing eczema, wheeze and sensitization
    Authors: T. R. Abrahamsson, M. Sandberg Abelius, A. Forsberg, B. Björkstén, M. C. Jenmalm
    Clinical & Experimental Allergy
  4. Th1 and Th2 Chemokines, Vaccine-Induced Immunity, and Allergic Disease in Infants After Maternal omega -3 Fatty Acid Supplementation During Pregnancy and Lactation
    Authors: Catrin Furuhjelm, Maria C Jenmalm, Karin Fälth-Magnusson, Karel Duchén
    Pediatric Research
  5. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
    Authors: Osei-Kumah A, Smith R, Clifton VL
    Cytokine, 2008-07-17;43(2):187-93.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  6. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006-06-16;120(2):220-31.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  7. Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission.
    Authors: Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA
    Parasitology, 2005-03-01;130(0):333-42.
    Species: Human
    Sample Types: Saliva
    Applications: ELISA Development
  8. Chemoattractant factors in breast milk from allergic and nonallergic mothers.
    Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
    Pediatr. Res., 2000-05-01;47(5):592-7.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

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Reviews for Human CCL11/Eotaxin Biotinylated Antibody

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Human CCL11/Eotaxin Biotinylated Antibody
By Anonymous on 11/16/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human
ELISA Human CCL11/Eotaxin Biotinylated Antibody BAF320