Human CCL1/I-309/TCA-3 Antibody

Catalog #: AF272
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF272
AF272-SP

Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCL1/I‑309 Antibody.

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Citations (3)

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Human CCL1/I-309/TCA-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL1/I‑309/TCA‑3 in direct ELISAs and Western blots. Neutralizes the biological activity of recombinant human CCL1. Does not block the biological activity of recombinant mouse TCA-3.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CCL1/I‑309/TCA‑3
Lys24-Lys96
Accession # P22362

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.15 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CCL1/I‑309/TCA‑3 (Catalog # 272-I)

Neutralization

Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND₅₀) is typically 0.4-2.0 µg/mL in the presence of 0.02 µg/mL Recombinant Human CCL1/I‑309/TCA‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCL1/I‑309 Antibody. Recombinant Human CCL1/I-309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I-309 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL1/I-309 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF272). The ND₅₀ is typically 0.4-2.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL1/I-309/TCA-3

Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV‑transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes.

CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The CCL1 sequence contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15‑16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.

References

Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci USA 89:2950.
Miller, M.D. et al. (1990) J. Immunol. 145:2737.

Entrez Gene IDs

6346 (Human); 20290 (Mouse)

Alternate Names

C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CCL1/I-309/TCA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Protumoral TSP50 Regulates Macrophage Activities and Polarization via Production of TNF-alpha and IL-1 beta, and Activation of the NF-kappa B Signaling Pathway
Authors: Cheng Yang, Dong-Mei Zhang, Zhen-Bo Song, Ya-Qin Hou, Yong-Li Bao, Lu-Guo Sun et al.
PLOS ONE
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
J. Clin. Invest., 2010-02-08;120(3):907-23.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
CC chemokine ligand 1 is released into the airways of atopic asthmatics.
Authors: Montes-Vizuet R, Vega-Miranda A, Valencia-Maqueda E, Negrete-Garcia MC, Velasquez JR, Teran LM
Eur. Respir. J., 2006-03-15;28(1):59-67.
Species: Human
Sample Types: BALF
Applications: Affinity Purification