Home / Cathepsin F / Human Cathepsin F Antibody

Human Cathepsin F Antibody

Catalog #: AF2075
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2075
AF2075-SP
Detection of Cathepsin F in Human Heart.
1 Image
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cathepsin F Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cathepsin F in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse Cathepsin F is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin F
Ala20-Asp484
Accession # Q9UBX1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Cathepsin F
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human heart
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cathepsin F, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of Cathepsin F in Human Heart. Cathepsin F was detected in immersion fixed paraffin-embedded sections of Human Heart using Goat Anti-Human Cathepsin F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2075) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin F

Cathepsin F is a lysosomal cysteine protease that participates in protein degradation and has been implicated in tumor progression.

Entrez Gene IDs
8722 (Human); 56464 (Mouse)
Alternate Names
Cathepsin F; CATSFEC 3.4.22.41; CTSF; EC 3.4.22

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human Cathepsin F Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Reduced expression of cathepsin F predicts poor prognosis in patients with clear cell renal cell carcinoma
    Authors: Zhou, X;Chen, H;Huang, D;Guan, G;Ma, X;Cai, W;Liao, J;Guan, T;
    Scientific reports
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Cathepsin F and Fibulin-1 as novel diagnostic biomarkers for brain metastasis of non-small cell lung cancer
    Authors: S Wei, W Liu, M Xu, H Qin, C Liu, R Zhang, S Zhou, E Li, Z Liu, Q Wang
    British Journal of Cancer, 2022-02-25;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Enhanced wound healing, kinase and stem cell marker expression in diabetic organ-cultured human corneas upon MMP-10 and cathepsin F gene silencing.
    Authors: Saghizadeh M, Epifantseva I, Hemmati D, Ghiam C, Brunken W, Ljubimov A
    Invest Ophthalmol Vis Sci, 2013-12-17;54(13):8172-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Adenovirus-driven overexpression of proteinases in organ-cultured normal human corneas leads to diabetic-like changes.
    Authors: Saghizadeh M, Kramerov AA, Yaghoobzadeh Y et al.
    Brain Res Bull
  5. Normalization of wound healing and stem cell marker patterns in organ-cultured human diabetic corneas by gene therapy of limbal cells.
    Authors: Saghizadeh M, Dib Cm, Brunken Wj et al.
    Exp. Eye Res.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Cathepsin F Antibody

There are currently no reviews for this product. Be the first to review Human Cathepsin F Antibody and earn rewards!

Have you used Human Cathepsin F Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review