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Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody

Catalog #: AF1049
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1049
AF1049-SP
Cathepsin A/Lysosomal Carboxypeptidase A in Human Prostate.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody Summary

Species Reactivity
Human
Specificity
Detects both pro and active forms of human and mouse Cathepsin A/Lysosomal Carboxypeptidase A in direct ELISAs and Western blots. In Western blots, it recognizes all forms of recombinant human Cathepsin A: single chain (55 kDa), heavy chain (32 kDa), and light chain (20 kDa). Also in Western blots, less than 1% cross-reactivity with recombinant human (rh) Cathepsin B, rhCathepsin C, rhCathepsin D, rhCathepsin L, rhCathepsin L2/V, rhCathepsin O, rhCathepsin S and rhCathepsin X/Z/P is observed.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Cathepsin A/Lysosomal Carboxypeptidase A (Catalog # 1049-SE)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Cathepsin A/Lysosomal Carboxypeptidase A antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

Cathepsin A/Lysosomal Carboxypeptidase A in Human Prostate. Cathepsin A/Lysosomal Carboxypeptidase A was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Cathepsin A/Lysosomal Carboxypeptidase A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1049) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Cathepsin A/Lysosomal Carboxypeptidase A

Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1). Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH. Also known as protective protein, its association with beta -galactosidase ( beta -gal) and neuraminidase is essential for beta -gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of Cathepsin A causes the lysosomal storage disorder galactosialidosis, characterized by a combined secondary deficiency of beta -gal and neuraminidase. Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including tachykinins, indicating that extralysosomal Cathepsin A plays a role in regulation of functions of these molecules (2). Cathepsin A is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds.

References
  1. Pshezhetsky, A.V. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 1923, Academic Press, San Diego.
  2. Hiraiwa, M. (1999) Cell. Mol. Life. Sci. 56:894.
Entrez Gene IDs
5476 (Human); 19025 (Mouse)
Alternate Names
beta-galactosidase 2; beta-galactosidase protective protein; Carboxypeptidase C; Carboxypeptidase L; Cathepsin A; cathepsin ANGBE; CTSA; EC 3.4.16; EC 3.4.16.5; GLB2; GSL; Lysosomal Carboxypeptidase A; lysosomal protective protein; PPCA; PPGBprotective protein for beta-galactosidase (galactosialidosis); Protective protein cathepsin A; Protective protein for beta-galactosidase

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Citations for Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion
    Authors: GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva
    Redox Biology, 2021-12-05;48(0):102205.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Dot Blot
  2. Cathepsin A contributes to left ventricular remodeling by degrading extracellular superoxide dismutase in mice
    Authors: M Hohl, M Mayr, L Lang, AG Nickel, J Barallobre, X Yin, T Speer, SR Selejan, C Goettsch, K Erb, C Fecher-Tro, JC Reil, B Linz, S Ruf, T Hübschle, C Maack, M Böhm, T Sadowski, D Linz
    J. Biol. Chem., 2020-07-09;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Lysates, Whole Tissue
    Applications: IHC, Western Blot
  3. Identification of PIKfyve kinase as a target in multiple myeloma
    Authors: C Bonolo De, YX Zhu, N Sepetov, S Romanov, LA Bruins, CX Shi, CK Stein, JL Petit, AN Polito, ME Sharik, EW Meermeier, GJ Ahmann, ID Lopez Arme, J Kruse, PL Bergsagel, M Chesi, N Meurice, E Braggio, AK Stewart
    Haematologica, 2019-10-03;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Cathepsin A is expressed in primary human antigen-presenting cells.
    Authors: Reich M, Spindler KD, Burret M, Kalbacher H, Boehm BO, Burster T
    Immunol. Lett., 2009-11-30;128(2):143-7.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  5. Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer's Disease.
    Authors: Armstrong A, Mattsson N, Appelqvist H et al.
    Neuromolecular Med.

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