Human BTN3A1/2/3 Alexa Fluor® 405-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB7136V-100UG
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Human BTN3A1/2/3 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human BTN3A1, BTN3A2 and BTN3A3 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 849203
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD277/BTN3A1
Met1-Gly254
Accession # O00481
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human peripheral blood lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BTN3A1/2/3

Butyrophilin 3A1 (BTN3A1), also known as CD277, has high structural homology to the B7 superfamily of proteins and is expressed in various immune cells such as T and NK cells. BTN3A1 enhances TCR-induced cytokine production and cell proliferation. Early T-cell activation events such as TCR-induced cell signaling are increased upon BTN3A1 engagement. The BTN3A1 co-stimulatory pathway may be involved in the regulation of various cell-mediated immune responses.

Long Name
Butyrophilin, Subfamily 3, Member A1/2/3
Alternate Names
BT3.1;BTF5;BTN3.1;BTN3A1;CD277; BTN3A1/2/3

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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