Human BAI2 Antibody

Catalog #: MAB9338 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB9338-100
MAB9338-SP

Detection of Human BAI2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BAI2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human BAI2 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2528B

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human BAI2
Phe21-Asp911
Accession # O60241-3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human BAI2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human BAI2. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human BAI2 Monoclonal Antibody (Catalog # MAB9338) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BAI2 at approximately 172 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of BAI2 in HEK293 Human Cell Line Transfected with Human BAI2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human BAI2 or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human BAI2 Monoclonal Antibody (Catalog # MAB9338) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAI2

Brain angiogenesis inhibitor 2 (BAI2), along with BAI1 and BAI3, form a subfamily of brain specific adhesion GPCRs. They are large, glycosylated molecules, approximately 180-220 kDa, and play a role in neuronal synapse formation and maintenance. Mature human BAI2 consists of a 903 amino acid (aa) N-terminal extracellular domain (ECD) containing four TSP1 domains, a hormone binding domain (HBD), a GAIN domain and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 410 aa C-terminal cytoplasmic domain (1). Within the N-terminal ECD, human BAI2 shares 96% aa sequence identity with both mouse and rat BAI2. BAI2 is expressed primarily in neurons and astrocytes of the hippocampus and cerebral cortex in the brain (2, 3). To become functional, BAI2 might require activation by cleavage at the GPS domain (4). BAI family members are known to bind the C1q-like complement (C1qL) family via thrombospondin type 1 repeats (TSRs) which are conserved between BAI members (5). BAI2 has been shown to suppress expression of VEGF leading to increased neurogenesis in the dentate gyrus of the hippocampus (6).

References