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Human ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody

Catalog #: AF4494
1 Citation Datasheet / COA / SDS
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AF4494
AF4494-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody Summary

Species Reactivity
Human
Specificity
Detects human ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant mouse ASAHL is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ASAHL/N‑acylethanolamine-hydrolyzing Acid A isoform 1
Ser29-Lys359 (Val151Ile)
Accession # Q02083
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4494‑AH)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4494-AH), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase

ASAHL (N-acylsphingosine-amidohydrolase-like; N acylethanolamine hydrolyzine acid amidase; also acid ceramidase like protein) is a 30 kDa member of the acid ceramidase family. It is a secreted lysosomal glycoprotein that cleaves fatty acid amides such as palmitoylethanolamine. Human ASAHL is synthesized as a 359 amino acid (aa) preproprecursor that contains a 28 aa signal sequence, a 96 aa prosegment, and a 30 31 kDa, 235 aa mature region that begins at Cys125. Multiple isoforms likely exist. One isoform shows a deletion of aa 324 359, while another has a two aa substitution for aa 198 359. The human proform (aa 29 359) is 78% aa identical to mouse ASAHL proform.

References
    Entrez Gene IDs
    27163 (Human); 67111 (Mouse); 497009 (Rat)
    Alternate Names
    Acid ceramidase-like protein; ASAHL; ASAH-like protein; EC 3.5.1.-; NAAA; N-acylethanolamine acid amidase; Nacylethanolaminehydrolyzing Acid Amidase; N-acylethanolamine-hydrolyzing acid amidase; N-acylsphingosine amidohydrolase (acid ceramidase)-like; N-acylsphingosine amidohydrolase-like; PLT

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    Citation for Human ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    1 Citation: Showing 1 - 1

    1. Ulcerative colitis impairs the acylethanolamide-based anti-inflammatory system reversal by 5-aminosalicylic acid and glucocorticoids.
      Authors: Suarez J, Romero-Zerbo Y, Marquez L, Rivera P, Iglesias M, Bermudez-Silva FJ, Andreu M, Rodriguez de Fonseca F
      PLoS ONE, 2012-05-25;7(5):e37729.
      Species: Human
      Sample Types: Tissue Homogenates, Whole Tissue
      Applications: IHC, Western Blot

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