Human Activin C beta C subunit Antibody

Catalog # Availability Size / Price Qty
AF1629
AF1629-SP
Human Activin C ELISA Standard Curve.
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Human Activin C beta C subunit Antibody Summary

Species Reactivity
Human
Specificity
Detects human Activin C beta C subunit in direct ELISAs.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Activin C
Gly237-Ser352
Accession # P55103
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Activin C beta C subunit Monoclonal Antibody (Catalog # MAB1629).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human Activin C ELISA Standard Curve. Recombinant Human Activin C protein was serially diluted 2-fold and captured by Mouse Anti-Human Activin C beta C subunit Monoclonal Antibody (Catalog # MAB1629) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Activin C beta C subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1629) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin C

Activins are dimers consisting of two beta subunits. Several types of beta subunits have been described. These beta subunits can also heterodimerize with a constant alpha subunit to form Inhibins. The beta c subunit homodimerizes to form Activin C and has also been reported to heterodimerize with the beta A subunit.

Entrez Gene IDs
3626 (Human); 16325 (Mouse)
Alternate Names
inhibin beta C chain; Activin C; IHBC; INHBC; inhibin, beta C

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