Human ACE Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 3.6 | 10.7 | 22.1 | 3.44 | 10.3 | 21.8 |
| Standard Deviation | 0.08 | 0.3 | 0.95 | 0.21 | 0.4 | 0.87 |
| CV% | 2.2 | 2.8 | 4.3 | 6.1 | 3.9 | 4 |
Serum, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 3.57 | 10.9 | 22.5 | 3.77 | 11.7 | 24.4 |
| Standard Deviation | 0.12 | 0.43 | 0.9 | 0.29 | 0.6 | 1.2 |
| CV% | 3.4 | 3.9 | 4 | 7.7 | 5.1 | 4.9 |
Recovery
The recovery of ACE spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 98 | 89-106 |
| Heparin Plasma (n=4) | 100 | 94-106 |
| Serum (n=4) | 99 | 91-106 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: ACE/CD143
ACE and ACE-2, two cell surface proteases, are important regulators of the renin-angiotensin system (RAS), which plays a key role in maintaining blood pressure homeostasis and fluid salt balance in mammals. Because of its location and specificity, ACE plays additional roles in immunity, reproduction and neuropeptide regulation. ACE exists in two isoforms. Somatic ACE comprises two highly similar protease domains whereas germinal ACE comprises a single protease domain. ACE-2 consists of a single protease domain and is an essential regulator of heart and lung function. It is also a cellular receptor for the Spike protein of SARS cornonavirus that causes severe acute respiratory syndrome.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human ACE Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Reduced blood pressure in sickle cell disease is associated with decreased angiotensin converting enzyme (ACE) activity and is not modulated by ACE inhibition
Authors: PL Brito, AF Dos Santos, H Chweih, ME Favero, EMF Gotardo, JAF Silva, FC Leonardo, CF Franco-Pen, MG de Oliveir, WA Ferreira, BC Zaidan, A Billis, G Baldanzi, DA Mashima, E Antunes, STO Saad, FF Costa, N Conran
PLoS ONE, 2022-02-03;17(2):e0263424.
Species: Human
Sample Types: Plasma
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RhoA-Rho associated kinase signaling leads to renin-angiotensin system imbalance and angiotensin converting enzyme 2 has a protective role in acute pulmonary embolism
Authors: X Xu, L Shi, X Ma, H Su, G Ma, X Wu, K Ying, R Zhang
Thromb. Res., 2019-02-15;176(0):85-94.
Species: Human
Sample Types: Plasma
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Associations of Angiotensin Converting Enzyme-1 and Angiotensin II Blood Levels and Cognitive Function
Authors: S Yasar, VR Varma, GC Harris, MC Carlson
J. Alzheimers Dis., 2018-01-01;0(0):.
Species: Human
Sample Types: Serum
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Circulating ACE is a predictor of weight loss maintenance not only in overweight and obese women, but also in men.
Authors: Wang P, Holst C, Wodzig W, Andersen M, Astrup A, van Baak M, Larsen T, Jebb S, Kafatos A, Pfeiffer A, Martinez J, Handjieva-Darlenska T, Kunesova M, Viguerie N, Langin D, Saris W, Mariman E
Int J Obes (Lond), 2012-01-24;36(12):1545-51.
Species: Human
Sample Types: Serum
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Soluble adhesion molecules and angiotensin-converting enzyme in dementia.
Authors: Nielsen HM, Londos E, Minthon L, Janciauskiene SM
Neurobiol. Dis., 2007-01-31;26(1):27-35.
Species: Human
Sample Types: Plasma
FAQs
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Is ACE-1 the same as ACE?
Yes. ACE-1 is used as an alternative name, but not as commonly as ACE and CD143.
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