HT 8-oxo-dG ELISA Kit II
For the detection and quantitation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in DNA, plasma, urine and saliva samples
HT 8-oxo-dG ELISA Kit II Summary
Quantifies 8-hydroxy-2'-deoxyguanosine (8-OHdG) in DNA, plasma, urine and saliva samples.Key Benefits
• Colorimetric, non-radioactive format
• High throughput 96 strip wells
• Dynamic range from 3.13 nM to 200 nM (0.89 ng/mL to 56.7 ng/mL)
• Sensitivity at 2 nM (0.57 ng/mL) 8-OHdG
Product Specifications
For the detection and quantitation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in DNA, plasma, urine and saliva samples.
Other Resources
4380-096-k_calculation_worksheet
Kit Contents
• 8-OHdG Standard, 20 µM
• 8-OHdG Monoclonal Antibody
• Goat anti-Mouse IgG-HRP
• DNase I (5 Units/µl)
• Alkaline Phosphatase (1 Unit/µl)
• Assay Dilutent
• 100X Cations
• Pre-coated 96-stripwell plate
• TACS-Sapphire
Specifications
Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Citations for HT 8-oxo-dG ELISA Kit II
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Acetyl Tributyl Citrate Exposure at Seemingly Safe Concentrations Induces Adverse Effects in Different Genders of Type 2 Diabetes Mice, Especially Brain Tissue
Authors: Zhang, Y;Zhang, Z;Zhu, S;Liu, L;Liu, X;Yang, X;
Toxics 2023-10-23
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Comparable Response Following Exposure to Biodiesel and Diesel Exhaust Particles in Advanced Multicellular Human Lung Models
Authors: Rahman, M;Upadhyay, S;Ganguly, K;Introna, M;Ji, J;Boman, C;Muala, A;Blomberg, A;Sandström, T;Palmberg, L;
Toxics 2023-06-14
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A coffee enriched with guarana, selenium, and l-carnitine (GSC) has nutrigenomic effects on oxi-inflammatory markers of relapsing-remitting multiple sclerosis patients: A pilot study
Authors: CF Teixeira, VF Azzolin, G Rodrigues, BO Turra, AO Alves, ACM Bressanim, LEL Canton, AC Vieira Dos, MH Mastella, F Barbisan, EE Ribeiro, T Duarte, MMMF Duarte, NCA Bonotto, DK Sato, IBM da Cruz
Multiple sclerosis and related disorders, 2023-01-13;71(0):104515. 2023-01-13
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In utero arsenic exposure increases DNA damage and gene expression changes in umbilical cord mesenchymal stem cells (UC-MSCs) from newborns as well as in UC-MSC differentiated hepatocytes
Authors: S Kantisin, K Chaisatra, P Hunsonti, V Parnlob, P Navasumrit, M Ruchirawat
Toxicology reports, 2022-09-07;9(0):1728-1741. 2022-09-07
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A Nested Case-Control Study of the Relationship between Salivary Inflammatory Mediators, Periodontal Parameters, and Preterm Birth in a Chinese Population
Authors: M Wu, C Ye, H Li, X Yang, S Zhu, F Zhou, Y Hao, S Chen, S Jiang
BioMed Research International, 2022-08-18;2022(0):8629680. 2022-08-18
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Monitoring DNA polymerase beta mitochondrial localization and dynamics
Authors: JK Horton, AK Janoshazi, CA Nadalutti, ML Zhao, DF Stefanick, SH Wilson
DNA repair, 2022-06-11;116(0):103357. 2022-06-11
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Degradation of the Escherichia coli Essential Proteins DapB and Dxr Results in Oxidative Stress, which Contributes to Lethality through Incomplete Base Excision Repair
Authors: CC Gruber, VMP Babu, K Livingston, H Joisher, GC Walker
MBio, 2022-02-08;0(0):e0375621. 2022-02-08
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Modulation of pancreatic cancer cell sensitivity to FOLFIRINOX through microRNA-mediated regulation of DNA damage
Authors: P Carotenuto, F Amato, A Lampis, C Rae, S Hedayat, MC Previdi, D Zito, M Raj, V Guzzardo, F Sclafani, A Lanese, C Parisi, C Vicentini, I Said-Hunti, JC Hahne, A Hallsworth, V Kirkin, K Young, R Begum, A Wotherspoo, K Kouvelakis, SX Azevedo, V Michalarea, R Upstill-Go, S Rao, D Watkins, N Starling, A Sadanandam, DK Chang, AV Biankin, NB Jamieson, A Scarpa, D Cunningham, I Chau, P Workman, M Fassan, N Valeri, C Braconi
Nature Communications, 2021-11-18;12(1):6738. 2021-11-18
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AKR1C1 alleviates LPS?induced ALI in mice by activating the JAK2/STAT3 signaling pathway
Authors: X Wang, B Yang, Y Li, J Luo, Y Wang
Molecular Medicine Reports, 2021-09-30;24(6):. 2021-09-30
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Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in�vivo cell engraftment
Authors: AM Pettinato, D Yoo, J VanOudenho, YS Chen, R Cohn, FA Ladha, X Yang, K Thakar, R Romano, N Legere, E Meredith, P Robson, M Regnier, JL Cotney, CE Murry, JT Hinson
Cell Reports, 2021-05-04;35(5):109088. 2021-05-04
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Diagnostic and prognostic ability of salivary MMP-9 for oral squamous cell carcinoma: A pre-/post-surgery case and matched control study
Authors: YJ Shin, H Vu, JH Lee, HD Kim
PLoS ONE, 2021-03-18;16(3):e0248167. 2021-03-18
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Impairment of Pol &beta-related DNA Base-excision Repair Leads to Ovarian Aging in Mice
Authors: K Hua, L Wang, J Sun, N Zhou, Y Zhang, F Ji, L Jing, Y Yang, W Xia, Z Hu, F Pan, X Chen, B Yao, Z Guo
Aging (Albany NY), 2020-11-20;12(0):. 2020-11-20
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Indole-6-Carboxaldehyde Isolated from Sargassum thunbergii (Mertens) Kuntze Prevents Oxidative Stress-Induced Cellular Damage in V79-4 Chinese Hamster Lung Fibroblasts through the Activation of the Nrf2/HO-1 Signaling Pathway
Authors: SO Kim, YH Choi
Cell Physiol Biochem, 2020-10-01;54(5):959-974. 2020-10-01
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Jianpi-yangwei decoction inhibits DNA damage repair in the drug resistance of gastric cancer by reducing FEN1 expression
Authors: W Huang, H Tang, F Wen, X Lu, Q Li, P Shu
BMC Complement Med Ther, 2020-06-26;20(1):196. 2020-06-26
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CoPP-Induced-Induced HO-1 Overexpression Alleviates Photoreceptor Degeneration With Rapid Dynamics: A Therapeutic Molecular Against Retinopathy
Authors: Y Tao, L Cai, D Zhou, C Wang, Z Ma, X Dong, G Peng
Invest. Ophthalmol. Vis. Sci., 2019-12-02;60(15):5080-5094. 2019-12-02
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Urinary Biomarkers of Inflammation and Oxidative Stress Are Elevated in Obese Children and Correlate with a Marker of Endothelial Dysfunction
Authors: V Selvaraju, P Ayine, M Fadamiro, JR Babu, M Brown, T Geetha
Oxid Med Cell Longev, 2019-10-16;2019(0):9604740. 2019-10-16
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Blood leukocyte LINE-1 hypomethylation and oxidative stress in knee osteoarthritis
Authors: N Teerawatta, W Udomsinpra, S Ngarmukos, A Tanavalee, S Honsawek
Heliyon, 2019-05-24;5(5):e01774. 2019-05-24
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Multi-walled carbon nanotubes: A cytotoxicity study in relation to functionalization, dose and dispersion.
Authors: Zhou L, Forman H, Ge Y, Lunec J
Toxicol In Vitro, 2017-05-05;42(0):292-298. 2017-05-05
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Effects of moderate alcohol consumption on gene expression related to colonic inflammation and antioxidant enzymes in rats.
Authors: Klarich D, Penprase J, Cintora P, Medrano O, Erwin D, Brasser S, Hong M
Alcohol, 2017-04-18;61(0):25-31. 2017-04-18
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Increased oxidative stress and cytokinesis-block micronucleus cytome assay parameters in pregnant women with gestational diabetes mellitus and gestational arterial hypertension.
Authors: Toljic M, Egic A, Munjas J, Karadzov Orlic N, Milovanovic Z, Radenkovic A, Vuceljic J, Joksic I
Reprod Toxicol, 2017-04-08;71(0):55-62. 2017-04-08
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NADPH oxidase 4 is a critical mediator in Ataxia telangiectasia disease.
Authors: Weyemi, Urbain, Redon, Christop, Aziz, Towqir, Choudhuri, Rohini, Maeda, Daisuke, Parekh, Palak R, Bonner, Michael, Arbiser, Jack L, Bonner, William
Proc Natl Acad Sci U S A, 2015-02-02;112(7):2121-6. 2015-02-02
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Role of Bacillus subtilis error prevention oxidized guanine system in counteracting hexavalent chromium-promoted oxidative DNA damage.
Authors: Santos-Escobar F, Gutierrez-Corona J, Pedraza-Reyes M
Appl Environ Microbiol, 2014-06-27;80(17):5493-502. 2014-06-27
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SOD3 acts as a tumor suppressor in PC-3 prostate cancer cells via hydrogen peroxide accumulation.
Authors: Kim J, Mizokami A, Shin M, Izumi K, Konaka H, Kadono Y, Kitagawa Y, Keller E, Zhang J, Namiki M
Anticancer Res, 2014-06-01;34(6):2821-31. 2014-06-01
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Smg1 haploinsufficiency predisposes to tumor formation and inflammation.
Authors: Roberts T, Ho U, Luff J, Lee C, Apte S, MacDonald K, Raggat L, Pettit A, Morrow C, Waters M, Chen P, Woods R, Thomas G, St Pierre L, Farah C, Clarke R, Brown J, Lavin M
Proc Natl Acad Sci U S A, 2012-12-31;110(4):E285-94. 2012-12-31
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Average Rating: 4 (Based on 4 Reviews)
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HT 8-oxo-dG ELISA Kit II
By Anonymous
on 09/06/2023
Catalog No. 4380-096-P (Contains 1 plate only)
LOT : P324064
Sample type being tested: Human Plasma
The standard curve came out well.
However, our plasma samples were diluted 1/10 suggested by the kit. Our OD values were out of range.
If you have enough plates, I would suggest to perform a standardization run, to determine optimal dilution for your samples first.
HT 8-oxo-dG ELISA Kit II
By Lindsie Morin
on 09/29/2020
Very sensitive assay that needs vigorous vortexing, but overall does produce good results.
HT 8-oxo-dG ELISA II Kit, 2 Plates
By Anonymous
on 12/17/2018
HT 8-oxo-dG ELISA II Kit, 2 Plates
By Anonymous
on 09/18/2018
