DuoSet ELISA Ancillary Reagent Kit 1
DuoSet ELISA Ancillary Reagent Kit 1 Summary
DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well assays. DuoSet Ancillary Kit 1 contains Reagent Diluent 1. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.
- Complete kit of supplemental supplies provides an economical way to assay five 96-well ELISA plates.
- For use with DuoSets requiring the following Reagent Diluent formulation: 1.4% delipidized bovine serum, 0.05% Tween® 20 in PBS, pH 7.2-7.4, 0.2 micron filtered.
- Pre-made buffers reduce preparation time and ensure optimal results.
- Clear Polystyrene Microplates (five 12x8-well strip microplates)
- ELISA Plate Sealers (24 plate sealers)
- Color Reagent A (1X, three 12.5 mL vials)
- Color Reagent B (1X, three 12.5 mL vials)
- Stop Solution (1X, three 11 mL vials)
- 20X PBS
- ELISA Plate-coating Buffer (1X, one 60 mL vial)
- Reagent Diluent Concentrate 1 (one 21 mL vial of a buffered protein base)
- Wash Buffer (25X, seven 21 mL vials)
- Reagent Diluent Concentrate 3 (5X, two 21 mL vials of a 25% Tween 20 solution in PBS).
For Use as the blocking buffer.
Specifications
Product Datasheets
Citations for DuoSet ELISA Ancillary Reagent Kit 1
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Cullin 4B Ubiquitin Ligase Is Important for Cell Survival and Regulates TGF-?1 Expression in Pleural Mesothelioma
Authors: Kreienbühl, J;Changkhong, S;Orlowski, V;Kirschner, MB;Opitz, I;Meerang, M;
International journal of molecular sciences 2023-08-29
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Identification of Potential microRNA Panels for Male Non-Small Cell Lung Cancer Identification Using Microarray Datasets and Bioinformatics Methods
Authors: A Harangu?, R Lajos, L Budisan, O Zanoaga, C Ciocan, C Bica, R Pirlog, I Simon, M Simon, C Braicu, I Berindan-N
Journal of personalized medicine, 2022-12-13;12(12):. 2022-12-13
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Eribulin normalizes pancreatic cancer-associated fibroblasts by simulating selected features of TGFbeta inhibition
Authors: T Luong, E Cukierman
BMC Cancer, 2022-12-02;22(1):1255. 2022-12-02
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Transforming growth factor-beta signaling modifies the hematopoietic acute inflammatory response to drive bone marrow failure
Authors: J Javier, A Hinge, J Bartram, J Xu, MD Filippi
Haematologica, 2022-06-01;107(6):1323-1334. 2022-06-01
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Effects of Short-Term Phosphate Loading on Aerobic Capacity under Acute Hypoxia in Cyclists: A Randomized, Placebo-Controlled, Crossover Study
Authors: K P?oszczyca, M Chalimoniu, I Przybylska, M Czuba
Nutrients, 2022-01-06;14(2):. 2022-01-06
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Latent TGFbeta-binding proteins regulate UCP1 expression and function via TGFbeta2
Authors: D Halbgebaue, J Roos, JB Funcke, H Neubauer, BS Hamilton, E Simon, EZ Amri, KM Debatin, M Wabitsch, P Fischer-Po, D Tews
Molecular Metabolism, 2021-09-01;53(0):101336. 2021-09-01
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Palladin isoforms 3 and 4 regulate cancer-associated fibroblast pro-tumor functions in pancreatic ductal adenocarcinoma
Authors: JI Alexander, DB Vendramini, R Francescon, T Luong, J Franco-Bar, N Shah, JC Gardiner, E Nicolas, KS Raghavan, E Cukierman
Scientific Reports, 2021-02-15;11(1):3802. 2021-02-15
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GLP-1 receptor agonist ameliorates experimental lung fibrosis
Authors: J Fandiño, L Toba, LC González-M, Y Diz-Chaves, F Mallo
Sci Rep, 2020-10-22;10(1):18091. 2020-10-22
FAQs
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View all ELISA FAQsReviews for DuoSet ELISA Ancillary Reagent Kit 1
Average Rating: 4.9 (Based on 8 Reviews)
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Very nice dose-response curve using ancillary kit. The kit is very stable.
works perfectly with il-1
Used with the Mouse TGF-beta 1 DuoSet ELISA. Performed with precision from one plate to the next. Instructions were complete and easy to follow.
Confusing reagent labels especially blocking buffer
instructions kind of confusing - have the directions are on the ancillary kit and the other are with the antibody kit. Also some of the buffers names need to be clearer and more consistent (e.g. blocking and coating). Results are good though so thats what matters.
