Cotton Rat IFN-alpha Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1082
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Cotton Rat IFN-alpha Biotinylated Antibody Summary

Species Reactivity
Cotton Rat
Specificity
Detects cotton rat IFN-alpha in Western blots. In this format, less than 1% cross-reactivity with recombinant human IFN-beta and recombinant cotton rat IFN-gamma is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant cotton rat IFN-alpha (R&D Systems, Catalog # 1082-CR)
Cys24-Lys190 with a Cys109Tyr substitution
Accession # AAL18816
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Cotton Rat IFN-alpha (Cys109Tyr) (Catalog # 1082-CR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha

Interferon-alpha, also known as leukocyte interferon, comprises a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R1) and a 125 kDa subunit (IFN-alpha R2) that is involved both in ligand-binding and signal transduction. IFN-alpha is expressed primarily by leukocytes upon activation by viruses, bacteria, cytokines and growth factors. IFN-alpha has been shown to have anti-viral and immunomodulatory activities on target cells. It can also inhibit cell proliferation as well as induce apoptosis (1, 2).

Cotton rat IFN-alpha 1 cDNA encodes a 189 amino acid (aa) residue precursor protein with a putative 23 aa signal sequence and a 166 aa mature protein. The protein contains five cysteines, four of which are involved in two intrachain disulfide bonds. Cotton rat IFN-alpha 1 shares 52%, 67%, 68% and 72% aa sequence identity to human, rat, hamster and mouse IFN-alpha 1, respectively.

References
  1. Domanski, P. and O.R. Colamonici (1996) Cytokine Growth Factor Rev. 7:143.
  2. Pestka, S. (2000) Biopolymers 55:254.
Long Name
Interferon alpha
Entrez Gene IDs
3440 (Human)
Alternate Names
IFNA; IFNA2; IFNA2b; IFNalpha; IFN-alpha; IFN-alphaA; INFA2; interferon alpha 2; LeIF D

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