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CometAssay Single Cell Gel Electrophoresis Assay-Silver

Catalog #: 4251-050-K
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
4251-050-K
Product Details
Citations (6)
FAQs
Reviews
Summary Product Datasheets

CometAssay Single Cell Gel Electrophoresis Assay-Silver Summary

Includes CometAssay reagents and components for the convenient silver staining of single cell gel electrophoresis samples.
 

Key Benefits

• Allows visualization by standard light microscopy
• Permanent staining for archiving samples
• Optimized for CometSlide staining

Designed specifically for use with the CometSlides to minimize unwanted background and the amount of hazardous waste generated by silver nitrate. The silver staining components may be purchased separately.

Product Specifications

CometAssay and FLARE

NOTE: Once reconstituted 2X Staining Reagent #4 is stable for 3 months at 4°C.

Kit Contents

• CometAssay Lysis Solution
• CometAssay LMAgarose
• CometSlides (2 Well, 25 Slides)
• 200 mM EDTA, pH 10
• 20X Staining Reagent #1
• 20X Staining Reagent #2
• 20X Staining Reagent #3
• 2X Staining Reagent #4
• 10X Fixation Additive
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended on the label.
Storage
Store the unopened product at room temperature. Do not use past expiration date.
Species
N/A

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

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Product Datasheet
COA

Citations for CometAssay Single Cell Gel Electrophoresis Assay-Silver

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Anticancer Activity of ?-6 Fatty Acids through Increased 4-HNE in Breast Cancer Cells
    Authors: C Bose, A Hindle, J Lee, J Kopel, S Tonk, PT Palade, SS Singhal, S Awasthi, SP Singh
    Cancers, 2021-12-20;13(24):.  2021-12-20
  2. Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma
    Authors: AE Gibson, C Yeung, SH Issaq, VJ Collins, M Gouzoulis, Y Zhang, J Ji, A Mendoza, CM Heske
    Oncogenesis, 2020-09-10;9(9):80.  2020-09-10
  3. Effect of iron overload from multi walled carbon nanotubes on neutrophil-like differentiated HL-60 cells
    Authors: Y Tabei, H Fukui, A Nishioka, Y Hagiwara, K Sato, T Yoneda, T Koyama, M Horie
    Sci Rep, 2019-02-18;9(1):2224.  2019-02-18
  4. Genomic stability, anti-inflammatory phenotype, and up-regulation of the RNAseH2 in cells from centenarians
    Authors: G Storci, S De Carolis, A Papi, MG Bacalini, N Gensous, E Marasco, A Tesei, F Fabbri, C Arienti, M Zanoni, A Sarnelli, S Santi, F Olivieri, E Mensà, S Latini, M Ferracin, S Salvioli, P Garagnani, C Franceschi, M Bonafè
    Cell Death Differ., 2019-01-08;0(0):.  2019-01-08
  5. HIV-1 induces telomerase activity in monocyte-derived macrophages, possibly safeguarding one of its reservoirs.
    Authors: Reynoso R, Wieser M, Ojeda D, Bonisch M, Kuhnel H, Bolcic F, Quendler H, Grillari J, Grillari-Voglauer R, Quarleri J
    J Virol, 2012-07-11;86(19):10327-37.  2012-07-11
  6. Elevated cAMP level attenuates 2-deoxy-d-ribose-induced oxidative damage in pancreatic beta-cells.
    Authors: Koh G, Suh KS, Chon S, Oh S, Woo JT, Kim SW, Kim JW, Kim YS
    Arch. Biochem. Biophys., 2005-04-09;438(1):70-9.  2005-04-09

FAQs

  1. How long can CometSlides™ be stored after electrophoresis?

    • Prior to staining, slides immersed in 70% ethanol for 5 minutes and then dried can be stored at room temperature for 1 year prior to staining. Permanent records can be created after staining for visualization by standard light microscopy using the CometAssay® Silver Staining Kit (Catalog # 4251-050-K).

  2. What can cause observation of artefacts during imaging of Comet data?

    • For optimal imaging, all cells should be in the same plane and this is achieved by completely drying the agarose. Artefacts may be introduced if the source cells used for analyis has any debris.  The debris should be allowed to settle before pipetting cells.  The LMagarose can also be the cause of artefacts.  To prevent debris from accumulating in the agar, melt it at 80-90 °C so it is runny, spin it at low speed and aliquot into sterile tubes in a hood. Use each aliquot for a single assay.

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