Canine CD34 PE-conjugated Antibody

Discontinued Product

FAB3346P has been discontinued.
View all CD34 products.
Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry.
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Product Details
Citations (2)
FAQs
Reviews

Canine CD34 PE-conjugated Antibody Summary

Species Reactivity
Canine
Specificity
Detects canine CD34 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 1H6
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant canine CD34
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD34 antibody in CLL-1390 Canine Cell Line antibody by Flow Cytometry. View Larger

Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry. CLL-1390 canine leukocytic round cell neoplasia cell line was stained with Mouse Anti-Canine CD34 PE-conjugated Monoclonal Antibody (Catalog # FAB3346P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Canine CD34 by Flow Cytometry View Larger

Detection of Canine CD34 by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine CD34 by Flow Cytometry View Larger

Detection of Canine CD34 by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD34

CD34 is a sialomucin molecule that is expressed on primitive hematopoietic stem cells and downregulated as they differentiate into mature cells. Although its precise function remains unknown, the pattern of expression of CD34 suggests that it plays a significant role in early hematopoiesis.

Entrez Gene IDs
947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
Alternate Names
CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1

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Citations for Canine CD34 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Immortalized Canine Adipose-Derived Mesenchymal Stem Cells as a Novel Candidate Cell Source for Mesenchymal Stem Cell Therapy
    Authors: Y Yasumura, T Teshima, T Nagashima, T Takano, M Michishita, Y Taira, R Suzuki, H Matsumoto
    International Journal of Molecular Sciences, 2023-01-23;24(3):.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
    BMC Vet Res, 2012-08-31;8(0):150.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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