The HT 8-oxo-dG ELISA is a plate-based immunoassay designed for detecting and quantifying 8-hydroxy-2’-deoxyguanosine (8-oxo-dG). 8-oxo-dG is a frequently used biomarker of oxidative DNA damage and oxidative stress. Reactive oxygen and nitrogen species generated by normal metabolic processes and a variety of environmental factors cause the hydroxylation of guanosine at the N7-C8 bond. 8-oxo-dG is unstable and, as a result, polymerases preferentially insert adenine opposite it. If the modified DNA is not repaired, the oxidative damaged adducts can lead to G-to-T transitions. Increased levels of 8-oxo-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension.
HT 8-oxo-dG ELISA Kit II (Catalog # 4380-096-K)
This kit is designed for measuring 8-oxo-dG in plasma, urine, and saliva samples. In addition, this kit provides cations, DNase I, and alkaline phosphatase for prepping DNA extracted from cultured cells, adherent cells, and tissue for use in the assay. This ELISA uses TACS-Sapphire™ as the substrate for HRP. Absorbance is measured at 450 nm using a standard microplate reader.
Kit Contents
Kit contents may vary slightly. Please refer to product-specific inserts for a complete list of contents for each individual assay.
- Pre-coated 96-stripwell Microplate
- 8-oxo-dG Monoclonal Detection Antibody
- HRP-conjugated Secondary Antibody
- 8-oxo-dG Standard
- Diluents & Buffers
- Colorimetric Substrate
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| Detection of 8-oxo-dG in Calf Thymus DNA using the HT 8-oxo-dG ELISA. Calf thymus DNA was untreated or treated with increasing concentrations of chromium (III) chloride (CrCl3), in the presence of 0.5 mM H2O2, to form 8-oxo-dG in the DNA. The reaction was terminated by ethanol precipitation, and the DNA was digested by DNase I (provided in kit) and alkaline phosphatase (provided in kit). Levels of 8-oxo-dG were measured using the HT 8-oxo-dG ELISA Kit II (Catalog # 4380-096-K). |
Supplemental Reagents
Anti-8-Hydroxyguanine (8-oxo-dG) Monoclonal Antibody (Catalog # 4354-MC-050)
Used to detect 8-oxo-dG (oxidative damage) by immunohistochemistry.
TACS-Sapphire is a trademark of Trevigen, Inc.