Quantikine ELISA Validation: Making the Industry Gold Standard

When selecting an ELISA kit, different users often have different criteria in making their choice, whether it is sensitivity, sample types, or ease-of-use. One universal criterion is that an ELISA must work the first time and every time you use it. R&D Systems has developed our
Quantikine^® ELISAs from raw materials that we make in-house, giving us unparalleled control over critical elements that affect your results and creating consistency of results over long periods of time. Whether you are breaking new ground or building on the work of others, you can trust that the results will be reproducible tomorrow, next week, and next year. Read below to see how we do it.

It Starts with Development

Manufacturing a quality ELISA kit begins with the reagents and optimization that takes place during the development of the assay. Antibodies and diluents are developed and tested in-house, and carefully selected to meet high performance criteria, stringent manufacturing guidelines, and quality control standards. Rigorous development and validation testing guarantees the ELISA will perform with the ultimate precision, specificity, accuracy, and sensitivity.

Confirmed Lot-to-Lot Consistency

All lots are tested to ensure low background, a linear standard curve, consistent assay sensitivity, and a broad dynamic standard curve range. A master calibrated standard is built during development, calibrated against highly purified material, and is used to build and qualify all future lots of standard. This guarantees that your sample values will not shift or change over time. Consistent control values established during assay development and validation ensure that sample data is comparable over time.

Precise Quantitation of the Target Analyte

Assay diluents are carefully optimized for each Quantikine^® ELISA Kit in order to minimize assay interference due to nonspecific binding, antibody interference, and cross-reactivity, which can be caused by assay surfaces or endogenous substances present in biological samples. Spike recovery and assay linearity experiments ensure that data are accurate for all validated sample types.

Accurate Detection of Natural Proteins

Antibody pairs recognize the supplied recombinant standard and the natural proteins in biological samples in a parallel manner, confirming that Quantikine^® ELISA Kits can be used to measure the relative mass values of the natural analyte. Protein standards are correlated to the NIBSC/WHO standard when available. R&D Systems has determined the ideal standard curve range for each assay, ensuring peak sensitivity and reproducibility of results.