How to Non-invasively Monitor Stem Cell Differentiation using Luminex® Technology

Joy Aho, PhD, Manager of Stem Cell Research and Development, Bio-Techne
Richard Fuerstenberg, Manager of Multiplex Development, Bio-Techne

In this webinar, Bio-Techne scientists, Richard Fuerstenberg and Joy Aho, discuss a novel method for monitoring stem cell pluripotency, expansion, and differentiation in vitro. This technology utilizes non-invasive sampling of cell culture supernatants to identify and follow changes in key secreted growth factors and cytokines. Using Proteome Profiler™ Antibody Arrays and Luminex^® Assays, researchers can quickly monitor changes in cell phenotype without sacrificing yield. These techniques can be used to minimize cross-experiment variability during differentiation as well as to identify key analytes that could be used to predict or direct cell expansion or differentiation.

Topics covered in this webinar include:

• Basics of Luminex^® Assays
• Disadvantages of current methods for monitoring cell differentiation
• Advantages of non-invasively monitoring stem cell differentiation
• Methods for semi-quantitative and quantitative profiling of stem cells in culture, including examples from:
  • Hepatocytes derived from embryonic and induced pluripotent stem cells
  • Cardiomyocytes derived from embryonic and induced pluripotent stem cells

Check out our Luminex^® HP Assay 101 Blog Series to learn the basics of Luminex^® Assays.
Read tips on how to standardize your stem cell differentiation.
View our full range of products for pluripotent stem cells.

 Luminex^® is a trademark of Luminex Corporation.