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CellXVivo Human B Cell Expansion Kit

Catalog #: CDK005
8 Citations 1 Review Datasheet / COA / SDS
For the proliferative expansion of human B cell populations
Catalog # Availability Size / Price Qty
CDK005
Light Microscopy of B Cells.
4 Images
Product Details
Procedure
Citations (8)
FAQs
Reviews (1)
Summary Product Datasheets Data Images Background

CellXVivo Human B Cell Expansion Kit Summary

Kit Summary

For the proliferative expansion of B cell populations.

Key Benefits

  • Yields a 3-5 fold expanded population of B cells
  • Provides optimized reagents needed to induce B cell proliferation
  • Utilizes validated and straightforward procedures
  • Does not require specialized instrumentation

 

Why Expand B Cells Ex Vivo?

B cells are a subset of lymphocytes that express immunoglobulins on their cell surface. The B cell population is a vital component of the humoral immune response. B cell activation leads to antigen uptake and presentation, affinity maturation and class switching of the immunoglobulins, and antibody secretion. B cells represent a small portion (~5%) of the peripheral lymphocyte population making it difficult to obtain sufficient B cell quantities for many research applications. The ability to expand B cell populations ex vivo is valuable to researchers who require B cells for downstream applications including high throughput B cell assays and ex vivo differentiation of T cells.

 

Kit Contents

This kit contains the following optimized reagents for the expansion of human B cell populations.

  • B Cell Expander Reagents
  • Reconstitution Buffers
  • Provides enough reagents for the 3-5 fold expansion of a starting population of 107 human B cells

Stability and Storage

Store the unopened kit at 2-8 °C. Do not use past the kit expiration date. Opened or reconstituted B Cell Expander 1, B Cell Expander 2, and B Cell Expander 3 should be stored at 2-8 °C under sterile conditions for up to 30 days or at -20 °C to -70 °C in a manual defrost freezer for up to 3 months.* Opened Reconstitution Buffer 1 and Reconstitution Buffer 2 should be stored at 2-8 °C under sterile conditions for up to 12 months.*

*Provided this is within the expiration date of the kit.

Specifications

Source
N/A
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Human

Product Datasheets

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Product Datasheet
COA
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Scientific Data

Cell Morphology Light Microscopy of B Cells. View Larger

Light Microscopy of B Cells. B cells were imaged at 20X magnification following isolation from PMBCs and (B) after 5 days in culture using reagents included in the Human B Cell Expansion Kit.

Flow Cytometry Detection of CD20 in Human B Cells. View Larger

Detection of CD20 in Human B Cells. Human B cells were expanded for 5 days using reagents included in the Human B Cell Expansion Kit. The cells were labeled with a PE-conjugated Mouse Anti-Human CD20 Monoclonal Antibody (R&D Systems, Catalog # FAB4225P; filled histogram) or a PE-conjugated Mouse IgG1 Isotype control (R&D Systems, Catalog # IC002P; open histogram).

Cell Differentiation/ Maturation Human B Cell Expansion.  View Larger

Human B Cell Expansion.  Human B cells were isolated from PMBCs and cultured for 5 days using reagents included in the Human B Cell Expansion Kit. B cell expansion was measured with Resazurin (R&D Systems, Catalog # AR002).

Assay Procedure

Refer to the product datasheet for complete product details.

 

Briefly, human B cell populations can be expanded using the following procedure:

  • Isolate human B cells from PBMC preparation
  • Culture B cells in B Cell Expander-supplemented media
  • Measure B cell expansion using Resazurin

View Graphic Procedure
 
Reagents Provided

Reagents Supplied in the CellXVivo™ Human B Cell Expansion Kit (Catalog # CDK005):

  • B Cell Expander 1
  • B Cell Expander 2
  • B Cell Expander 3
  • Reconstitution Buffer 1
  • Reconstitution Buffer 2

 

Other Supplies Required

Reagents

  • MagCellect™ Human B Cell Isolation Kit (R&D Systems, Catalog # MAGH103, or equivalent).
  • Resazurin (R&D Systems, Catalog # AR002, or equivalent).
  • Ficoll-Hypaque™
  • RPMI 1640
  • L-Glutamine
  • Penicillin
  • Streptomycin
  • Fetal Bovine Serum (FBS)
  • beta-Mercaptoethanol

Equipment

  • Tissue culture flasks and/or plates
  • Inverted microscope
  • Hemocytometer
  • 37 °C and 5% CO2 humidified cell culture incubator
  • Benchtop centrifuge
  • Pipettes and pipette tips

 

Procedure Overview

R&D Systems Protocol for Human B Cell expansion

Isolate PBMCs from human blood.

Isolate PBMCs from human blood

Isolate human B cells from a PBMC preparation (e.g., using magnetic cell selection).

Isolate human B Cells from PBMCs

Perform a cell count.

Perform a cell count

Culture 2 x 105 cells/mL in media supplemented with reconstituted B Cell Expanders 1-3.

Incubate the cells for 5 days.

Measure B cell expansion using Resazurin.

Culture cells in media

Citations for CellXVivo Human B Cell Expansion Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Triple targeting of RSK, AKT, and S6K as pivotal downstream effectors of PDPK1 by TAS0612 in B-cell lymphomas
    Authors: Katsuragawa-Taminishi, Y;Mizutani, S;Kawaji-Kanayama, Y;Onishi, A;Okamoto, H;Isa, R;Mizuhara, K;Muramatsu, A;Fujino, T;Tsukamoto, T;Shimura, Y;Taniwaki, M;Miyagawa-Hayashino, A;Konishi, E;Kuroda, J;
    Cancer science  2023-10-15
  2. Targeting CD74 in B-cell non-Hodgkin lymphoma with the antibody-drug conjugate STRO-001
    Authors: X Li, C Abrahams, A Yu, M Embry, R Henningsen, V DeAlmeida, S Matheny, T Kline, A Yam, R Stafford, T Hallam, M Lupher, A Molina
    Oncotarget, 2023-01-12;14(0):1-13.  2023-01-12
  3. The Rationale for the Dual-Targeting Therapy for RSK2 and AKT in Multiple Myeloma
    Authors: R Isa, M Horinaka, T Tsukamoto, K Mizuhara, Y Fujibayash, Y Taminishi-, H Okamoto, S Yasuda, Y Kawaji-Kan, Y Matsumura-, S Mizutani, Y Shimura, M Taniwaki, T Sakai, J Kuroda
    International Journal of Molecular Sciences, 2022-03-08;23(6):.  2022-03-08
  4. Tumor-B-cell interactions promote isotype switching to an immunosuppressive IgG4 antibody response through upregulation of IL-10 in triple negative breast cancers
    Authors: NJ Toney, LM Opdenaker, K Cicek, L Frerichs, CR Kennington, S Oberly, H Archinal, R Somasundar, J Sims-Mourt
    Journal of Translational Medicine, 2022-03-07;20(1):112.  2022-03-07
  5. IgA transcytosis and antigen recognition govern ovarian cancer immunity
    Authors: S Biswas, G Mandal, KK Payne, CM Anadon, CD Gatenbee, RA Chaurio, TL Costich, C Moran, CM Harro, KE Rigolizzo, JA Mine, J Trillo-Tin, N Sasamoto, KL Terry, D Marchion, A Buras, RM Wenham, X Yu, MK Townsend, SS Tworoger, PC Rodriguez, AR Anderson, JR Conejo-Gar
    Nature, 2021-02-03;0(0):.  2021-02-03
  6. B cell-specific XIST complex enforces X-inactivation and restrains atypical B cells
    Authors: B Yu, Y Qi, R Li, Q Shi, A Satpathy, HY Chang
    bioRxiv : the preprint server for biology, 2021-01-05;0(0):.  2021-01-05
  7. BAFF-driven NLRP3 inflammasome activation in B cells
    Authors: KH Lim, LC Chen, K Hsu, CC Chang, CY Chang, CW Kao, YF Chang, MC Chang, CG Chen
    Cell Death Dis, 2020-10-01;11(9):820.  2020-10-01
  8. A DNA Vaccine Protects Human Immune Cells against Zika Virus Infection in Humanized Mice
    Authors: G Yi, X Xu, S Abraham, S Petersen, H Guo, N Ortega, P Shankar, N Manjunath
    EBioMedicine, 2017-10-06;25(0):87-94.  2017-10-06

FAQs

  1. Can the CellXVivo Human B Cell Expansion Kit, catalog # CDK005, be used for expansion of memory B cells?

    • Based on the components used in the CDK005 kit, the kit is expected to expand total B cells and is not specialized for memory B cells.

  2. Will ExCellerate B Cell Media, Xeno-Free, Catalog # CCM031, promote expansion of B cell clones without addition of growth factors?

    • Catalog # CCM031 must be supplemented with growth factors in order to use it for expansion of B cell clones. Information on appropriate growth factor cocktails are available in published literature. Here are two references:

      1. LeBien, T.W. and T.F. Tedder. (2008) Blood 112:1570.

      2. Basso, K. and R. Dalla-Favera (2015) Nat. Rev. Immunol. 15:172.

  3. Can CellXVivo Human B cells expansion kit, Catalog # CDK005, also promote B cell Maturation (differentiation into plasma cells)?

    • Catalog # CDK005 has only been tested for B cell expansion. This kit has not been tested for B cell Maturation.

View all Cell Culture Product FAQs

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CellXVivo Human B Cell Expansion Kit
By Leslie Priddy on 04/04/2018