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Human RANK/TNFRSF11A Antibody

Catalog #: MAB683
9 Citations Datasheet / COA / SDS
Clone 80704 was used by HLDA to establish CD designation

Discontinued Product

MAB683 has been discontinued.
View all RANK/TNFRSF11A products.
Detection of RANK/TNFRSF11A in A431 Human Cell Line by Flow Cytometry.
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Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RANK/TNFRSF11A Antibody Summary

Species Reactivity
Human
Specificity
Detects human RANK/TNFRSF11A in ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLymphotoxin beta R, rhNGF R, rhOPG, rhTAJ, rhTNF RI, rhTNF RII, or recombinant mouse (rm) OX40, and rmRANK is observed.
Source
Monoclonal Mouse IgG1 Clone # 80704
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RANK/TNFRSF11A. extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human RANK/TNFRSF11A Fc Chimera (Catalog # 683-RK)
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human RANK/TNFRSF11A Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human RANK/TNFRSF11A Biotinylated Antibody (Catalog # BAF683)

Standard: Recombinant Human RANK/TNFRSF11A Fc Chimera Protein, CF (Catalog # 683-RK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of RANK/TNFRSF11A antibody in A431 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of RANK/TNFRSF11A in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human RANK/TNFRSF11A Monoclonal Antibody (Catalog # MAB683, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RANK/TNFRSF11A

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR]) and TNFRSF11A is a member of the tumor necrosis factor receptor family. The full length human RANK cDNA encodes a type I transmembrane protein of 616 amino acids with a predicted 184 amino acid extracellular domain and a 383 amino acid cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains. RANK is widely expressed with highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

References
  1. Anderson, D.M. et al. (1997) Nature 390:175.
  2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
Long Name
Receptor Activator of NF-kappa-B
Entrez Gene IDs
8792 (Human); 21934 (Mouse)
Alternate Names
CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANK; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator

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Citations for Human RANK/TNFRSF11A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Receptor Activator of NF-kappaB (RANK) Confers Resistance to Chemotherapy in AML and Associates with Dismal Disease Course
    Authors: KL Clar, LM Weber, BJ Schmied, JS Heitmann, M Marconato, C Tandler, P Schneider, HR Salih
    Cancers, 2021-12-04;13(23):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Caveolin?1 enhances RANKL?induced gastric cancer cell migration
    Authors: Y Wang, Y Song, X Che, L Zhang, Q Wang, X Zhang, J Qu, Z Li, L Xu, Y Zhang, Y Fan, K Hou, Y Liu, X Qu
    Oncol. Rep., 2018-07-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Characterization and application of two RANK-specific antibodies with different biological activities.
    Authors: Chypre M, Seaman J, Cordeiro O, Willen L, Knoop K, Buchanan A, Sainson R, Williams I, Yagita H, Schneider P, Mueller C
    Immunol Lett, 2016-01-07;171(0):5-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Langerhans cell precursors acquire RANK/CD265 in prenatal human skin.
    Authors: Schoppl A, Botta A, Prior M, Akgun J, Schuster C, Elbe-Burger A
    Acta Histochem, 2015-02-24;117(4):425-30.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Receptor activator for NF-kappaB ligand in acute myeloid leukemia: expression, function, and modulation of NK cell immunosurveillance.
    Authors: Schmiedel B, Nuebling T, Steinbacher J, Malinovska A, Wende C, Azuma M, Schneider P, Grosse-Hovest L, Salih H
    J Immunol, 2012-12-14;190(2):821-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. RANKL expression, function, and therapeutic targeting in multiple myeloma and chronic lymphocytic leukemia.
    Authors: Schmiedel B, Scheible C, Nuebling T, Kopp H, Wirths S, Azuma M, Schneider P, Jung G, Grosse-Hovest L, Salih H
    Cancer Res, 2012-11-08;73(2):683-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Regulatory T cells in the bone marrow microenvironment in patients with prostate cancer.
    Authors: Zhao E, Wang L, Dai J, Kryczek I, Wei S, Vatan L, Altuwaijri S, Sparwasser T, Wang G, Keller E, Zou W
    Oncoimmunology, 2012-03-01;1(2):152-161.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Epidermal receptor activator of NF-kappaB ligand controls Langerhans cells numbers and proliferation.
    Authors: Barbaroux JB, Beleut M, Brisken C, Mueller CG, Groves RW
    J. Immunol., 2008-07-15;181(2):1103-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  9. High expression levels of receptor activator of nuclear factor-kappa B ligand associated with human chronic periodontitis are mainly secreted by CD4+ T lymphocytes.
    Authors: Vernal R, Dutzan N, Hernandez M, Chandia S, Puente J, Leon R, Garcia L, Del Valle I, Silva A, Gamonal J
    J. Periodontol., 2006-10-01;77(10):1772-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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