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Human BCAM Antibody

Catalog #: MAB1481
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1481
MAB1481-SP
Detection of BCAM in Huh‑7 Human Cell Line by Flow Cytometry.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BCAM Antibody Summary

Species Reactivity
Human
Specificity
Detects human BCAM in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhEpCAM, recombinant mouse (rm) MAdCAM-1, rhMCAM, rhNCAM-L1, rmOCAM, or rmTROP‑2 is observed.
Source
Monoclonal Mouse IgG2A Clone # 87207
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BCAM
Glu32-Ala547
Accession # CAA58449
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human BCAM Fc Chimera (Catalog # 148-BC)
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human BCAM Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human BCAM Biotinylated Antibody (Catalog # BAF148)

Standard: Recombinant Human BCAM Fc Chimera Protein, CF (Catalog # 148-BC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of BCAM antibody in Huh-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of BCAM in Huh‑7 Human Cell Line by Flow Cytometry. Huh-7 human hepatoma cell line was stained with Mouse Anti-Human BCAM Monoclonal Antibody (Catalog # MAB1481, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCAM

Basal-Cell Adhesion Molecule (BCAM) and Lutheran blood group glycoprotein (LU) are two alternatively spliced variants of a single immunoglobulin superfamily (IgSF) protein that differ in the length of their cytoplasmic tails. BCAM cDNA encodes a 628 amino acid (aa) residues precursor protein with a putative 31 aa signal peptide, a 597 aa extracellular domain containing three C2 type and two V-type Ig-like domains, a 21 aa transmembrane domain, and a 19 aa cytoplasmic domain. Compared to the 40 aa cytoplasmic domain present in LU, the BCAM cytoplasmic tail lacks the putative Src homology 3 (SH3) binding site that may be involved in mediating intracellular signaling. BCAM/LU has wide tissue distribution and is expressed on erythrocytes, the endothelium of blood vessels and on the basal layer of cells in the epithelia. The expression of BCAM/LU in normal tissues is higher in fetal versus adult tissues. BCAM/LU expression is also upregulated in sickle cell disease red blood cells, in activated keratinocytes and following malignant transformation in some cell types in vivo and in vitro. BCAM/LU has been shown to be an adhesion molecule that binds laminin, a basement membrane protein involved in cell differentiation, adhesion, migration and proliferation.

References
  1. Campbell, I.G. et al. (1994) Cancer Research 54:5761.
  2. Parsons, S.F. et al. (1995) Proc. Natl., Acad. Sci. USA, 92:5496.
  3. Udani, M. et al. (1998) 101:2550.
  4. Schon, M. et al. (2000) J. Invest. Dermatol, 115:1047.
Long Name
Basal Cell Adhesion Molecule
Entrez Gene IDs
4059 (Human); 57278 (Mouse)
Alternate Names
antigen identified by monoclonal F8; Auberger B antigen; basal cell adhesion molecule (Lu and Au blood groups); basal cell adhesion molecule (Lutheran blood group); basal cell adhesion molecule; B-CAM cell surface glycoprotein; BCAM; B-cell adhesion molecule; CD239 antigen; CD239; F8/G253 antigen; glycoprotein 95kDa; LUAU; Lutheran antigen; Lutheran blood group (Auberger b antigen included); Lutheran blood group glycoprotein; MSK19

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Citations for Human BCAM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration
    Authors: Zhenyu Cai, Anling Zhang, Swati Choksi, Weihua Li, Tao Li, Xue-Min Zhang et al.
    Cell Research
  2. An Anti-Human Lutheran Glycoprotein Phage Antibody Inhibits Cell Migration on Laminin-511: Epitope Mapping of the Antibody
    Authors: Y Enomoto-Ok, Y Maeda, N Harashima, Y Sugawara, F Katagiri, K Hozumi, KM Hui, M Nomizu, Y Ito, Y Kikkawa
    PLoS ONE, 2017-01-06;12(1):e0167860.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Hydroxycarbamide decreases sickle reticulocyte adhesion to resting endothelium by inhibiting endothelial lutheran/basal cell adhesion molecule (Lu/BCAM) through phosphodiesterase 4A activation.
    Authors: Chaar V, Laurance S, Lapoumeroulie C, Cochet S, De Grandis M, Colin Y, Elion J, Le Van Kim C, El Nemer W
    J Biol Chem, 2014-03-10;289(16):11512-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. The lutheran/basal cell adhesion molecule promotes tumor cell migration by modulating integrin-mediated cell attachment to laminin-511 protein.
    Authors: Kikkawa Y, Ogawa T, Sudo R, Yamada Y, Katagiri F, Hozumi K, Nomizu M, Miner J
    J Biol Chem, 2013-09-13;288(43):30990-1001.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. An antibody to the lutheran glycoprotein (Lu) recognizing the LU4 blood type variant inhibits cell adhesion to laminin alpha5.
    Authors: Kikkawa Y, Miwa T, Tohara Y, Hamakubo T, Nomizu M
    PLoS ONE, 2011-08-12;6(8):e23329.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Blocking, Epitope Mapping
  6. Basal cell adhesion molecule promotes metastasis‐associated processes in ovarian cancer
    Authors: Suresh Sivakumar, Sonja Lieber, Damiano Librizzi, Corinna Keber, Leah Sommerfeld, Florian Finkernagel et al.
    Clinical and Translational Medicine

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