Human IL-17C Antibody Summary
His19-Val197
Accession # Q9P0M4.1
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Human IL-17C Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑17C in Human Crohn's Intestine. IL‑17C was detected in immersion fixed paraffin-embedded sections of human crohn's intestine using Mouse Anti-Human IL‑17C Monoclonal Antibody (Catalog # MAB1234) at 10 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of IL‑17C in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human IL-17C Monoclonal Antibody (Catalog # MAB1234, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17C
The Interleukin-17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL‑17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL‑17 RE, have also been reported (1‑4). Human IL-17C cDNA encodes a 197 amino acid (aa) residues protein with a putative 18 aa signal peptide (5). IL-17C shares from 15%‑30% aa sequence identity with other IL-17 family members. Human and mouse IL-17C also share 83% aa sequence identity. IL-17C has a very restricted expression pattern and was detected as a rare expressed sequence tag (EST) sequence in an adult prostate and fetal kidney libraries (2). IL-17C has been shown to stimulate the release of TNF-alpha and IL-1 beta from the monocytic cell line THP-1, a property it shares with IL-17B (5, 6).
- Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
- Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
- Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
- Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
- Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
- Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
Product Datasheets
Citations for Human IL-17C Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Unveiling the Ability of Witch Hazel (Hamamelis virginiana L.) Bark Extract to Impair Keratinocyte Inflammatory Cascade Typical of Atopic Eczema
Authors: S Piazza, G Martinelli, A Magnavacca, M Fumagalli, C Pozzoli, M Terno, L Canilli, M Angarano, N Maranta, M Dell'Agli, E Sangiovann
International Journal of Molecular Sciences, 2022-08-17;23(16):.
Species: Human
Sample Types: Cell Culture Superantes
Applications: ELISA Capture -
Activity-dependent synaptic Wnt release regulates hippocampal long term potentiation.
Authors: Chen J, Park CS, Tang SJ
J. Biol. Chem., 2006;281(17):11910-6.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
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