Recombinant Human EDA-A2 Protein

Carrier Free

Catalog # Availability Size / Price Qty
922-ED-010/CF

With Carrier

Catalog # Availability Size / Price Qty
922-ED-010
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (3)
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Recombinant Human EDA-A2 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human EDA2R/TNFRSF27/XEDAR Fc Chimera (Catalog # 1093-XD) is coated at 0.1  μg/mL (100 μL/well), the concentration of Recombinant Human EDA-A2/Ectodysplasin A2 that produces 50% of the optimal binding response is 3-15 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human EDA-A2/Ectodysplasin A2 protein
MHHHHHHHHHH GGGSGGGSGGGS IEGR Human EDA-A2
(Ala179-Ser389)
Accession # AAC77371
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
24.7 kDa
SDS-PAGE
28-38 kDa, reducing conditions

Product Datasheets

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922-ED (with carrier)

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922-ED/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

922-ED

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

922-ED/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EDA-A2/Ectodysplasin A2

EDA-A2, a product of the EDA gene (also called Tabby), is a type II transmembrane protein that is a member of the TNF Superfamily (TNFSF). Human EDA-A2 is a 389 amino acid (aa) protein with a predicted N-terminal 39 aa cytoplasmic domain, a 22 aa transmembrane domain and a C-terminal 328 aa extracellular domain. The extracellular domains of human and mouse EDA-A2 share approximately 94% identity. Within the TNFSF, EDA-A2 shares the highest homology with EDA-A1, the other product of the EDA gene. EDA-A2 and EDA-A1 are splice variants of EDA which differ by only two amino acids. EDA-A1 contains two additional amino acids, Glu 308 and Val 309. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, a member of the TNF Receptor Superfamily (TNFRSF), whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF family member with high homology to EDAR. Mutations in EDA-A1, EDA-A2, EDAR and XEDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene.

References
  1. Bayes, M. et al. (1998) Hum. Mol. Genet. 7:1661.
  2. Kere, J. et al. (1996) Nat. Genet. 13:409.
  3. Monreal, A.W. et al. (1998) Am. J. Hum. Genet. 63:380.
  4. Headon, D.J. and P.A. Overbeek (1999) Nat. Genet. 22(4):370.
  5. Kumar, A. et al. (2000) J. Biol. Chem. 276(4):2668.
  6. Monreal, A.W. et al. (1999) Nat. Genet. 22(4):366.
  7. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94(24):13069.
  8. Yan, M. et al. (2000) Science 290(5491):523.
Entrez Gene IDs
1896 (Human); 13607 (Mouse)
Alternate Names
ECTD1;Ectodysplasin-A;ED1;ED1-A1;ED1-A2;EDA1;EDA2;EDA-A1;EDA-A2;HED;HED1;ODT1;STHAGX1;TNLG7C;XHED;XLHED; EDAA2; EDA-A2

Citations for Recombinant Human EDA-A2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. EDA2R-NIK signalling promotes muscle atrophy linked to cancer cachexia
    Authors: Bilgic, SN;Domaniku, A;Toledo, B;Agca, S;Weber, BZC;Arabaci, DH;Ozornek, Z;Lause, P;Thissen, JP;Loumaye, A;Kir, S;
    Nature
    Species: Mouse, Human
    Sample Types: Transduced Whole Cells, Whole Cells
    Applications: Bioassay
  2. An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
    Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
    MAbs, 2018-03-05;0(0):1-43.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  3. p53-dependent transcriptional regulation of EDA2R and its involvement in chemotherapy-induced hair loss.
    Authors: Brosh R, Sarig R, Natan EB, Molchadsky A, Madar S, Bornstein C, Buganim Y, Shapira T, Goldfinger N, Paus R, Rotter V
    FEBS Lett., 2010-04-29;584(11):2473-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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