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Recombinant Human Pentraxin 3/TSG-14 Protein

Catalog #: 1826-TS
14 Citations 1 Review Datasheet / COA / SDS
Newer Version Available: 10292-TS
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Discontinued Product

1826-TS has been discontinued and is replaced by 10292-TS.

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Citations (14)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Pentraxin 3/TSG-14 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhC1q at 5 µg/mL (100 µL/well) can bind rhPentraxin 3 with a linear range of 0.08-5 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human Pentraxin 3/TSG-14 protein
Glu18-Ser381, with a C-terminal 6-His tag
Accession #
P26022
N-terminal Sequence
Analysis
Glu18
Structure / Form
Multimer consisting of as many as ten non-covalently and covalently linked subunits
Predicted Molecular Mass
41 kDa (monomer)
SDS-PAGE
41-50 kDa, reducing conditions

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1826-TS (with carrier)

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1826-TS/CF (carrier free)

Discontinued Product

1826-TS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), TSG-14, was initially identified as a TNF-alpha or IL-1 beta inducible gene (1-3). It belongs to the pentraxin family, which was named originally for the homo-pentameric structure formed by its members (4). The pentraxin family is divided into two subfamilies: the “short” and the “long” pentraxins with approximate molecular weights of 25 kDa and 50 kDa, respectively. TSG-14 is a member of the long pentraxin subfamily, which also includes the Xenopus laevis XL-PXN1, the guinea pig apexin/p50, the rat neuronal pentraxin I (NPI) and NPR, the human neuronal pentraxin II (NPTX2) and the human neuronal activity-related pentraxin (5).

Mature secreted TSG-14 contains a pentaxin-like domain at its carboxy-terminus that shares 23-28% amino acid (aa) sequence similarity to C-reactive protein (CRP) and serum amyloid P component (SAP), which belongs to the short pentraxin subfamily. However, the N-terminal sequence of TSG-14 does not share aa sequence homology with any of the “short” pentaxins (3). Unlike CRP and SAP, which forms pentamers only, TSG-14 forms both pentameric and higher ordered oligomers (5). Similarly to CRP and SAP, TSG-14 binds to the complement cascade component C1q (6). However, TSG-14 does not bind to phosphoethanolamine, phosphocholine, or high pyruvate agarose, which are known ligands for CRP and SAP. TSG-14 is a marker of the acute phase response and is highly expressed in advanced atherosclerotic plaques (12). While CRP and SAP are primarily produced in the liver, TSG-14 expression is strongly upregulated by TNF-alpha, IL-1 beta, and bacterial LPS in peripheral fibroblasts, endothelial cells, and macrophages (7). At the amino acid level, human and mouse TSG-14 share 88% aa sequence homology (8). TSG-14 concentration is elevated in the joint fluid of patients with rheumatoid arthritis (RA), indicating that TSG-14 may be a potential mediator of immune response (9).
TSG-14 may also function in the regulation of the uptake and clearance of apoptotic cells by dendritic cells (10). In vivo study showed that TSG-14 transgenic mice are more resistant to sepsis and endotoxemia compared to wild type during the inflammatory injury (11). Increased expression of TSG-14 may enhance the immune response to protect the host from infection.

References
  1. Lee, T.H. et al. (1990) Mol. Cell. Biol. 10:1982.
  2. Breviario, F. et al. (1992) J. Biol. Chem. 267:22190.
  3. Lee, G.W. et al. (1993) J. Immunol. 150:1804.
  4. Osmand, A.P. et al. (1977) Proc. Natl. Acad. Sci. USA 74:739.
  5. Goodman A.R. et al. (1996) Cytokine & Growth Factor Reviews 7:191.
  6. Bottazzi, B. et al. (1997) J. Biol. Chem. 272:32817.
  7. Introna, M. et al. (1996) Blood 87:1862.
  8. Altmeyer, A. et al. (1995) J. Biol. Chem. 270:25584.
  9. Luchetti, M.M. et al. (2000) Clin. Exp. Immunol. 119:196.
  10. Rovere, P. et al. (2000) Blood 96:4300.
  11. Dias, A.A.M. et al. (2001) J. Leukocyte Biol. 69:928.
  12. Rolph, M.S. et al. (2002) Arterioscler. Throm. Vasc. Biol. 22:e10-4.
Long Name
Tumor Necrosis Factor Stimulated Gene 14
Entrez Gene IDs
5806 (Human); 19288 (Mouse)
Alternate Names
alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14

Citations for Recombinant Human Pentraxin 3/TSG-14 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Hepatocyte growth factor derived from senescent cells attenuates cell competition-induced apical elimination of oncogenic cells
    Authors: N Igarashi, K Miyata, TM Loo, M Chiba, A Hanyu, M Nishio, H Kawasaki, H Zheng, S Toyokuni, S Kon, K Moriyama, Y Fujita, A Takahashi
    Oncogene, 2022-07-18;13(1):4157.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Inflamm-Aging Is Associated with Lower Plasma PTX3 Concentrations and an Impaired Capacity of PBMCs to Express hTERT following LPS Stimulation
    Authors: AL Slusher, TM Zúñiga, EO Acevedo
    Mediators Inflamm., 2019-09-12;2019(0):2324193.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Transcriptome analysis of human monocytic cells infected with Burkholderia species and exploration of pentraxin-3 as part of the innate immune response against the organisms
    Authors: SA Aschenbroi, ER Lafontaine, MC Lopez, HV Baker, RJ Hogan
    BMC Med Genomics, 2019-09-06;12(1):127.
    Species: Bacteria - Burkholderia mallei
    Sample Types: Bacteria
    Applications: Bioassay
  4. Monocyte differentiation and macrophage priming are regulated differentially by pentraxins and their ligands
    Authors: D Pilling, E Galvis-Car, TR Karhadkar, N Cox, RH Gomer
    BMC Immunol., 2017-06-15;18(1):30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. FHR-1 Binds to C-Reactive Protein and Enhances Rather than Inhibits Complement Activation
    Authors: ÁI Csincsi, Z Szabó, Z Bánlaki, B Uzonyi, M Cserhalmi, É Kárpáti, A Tortajada, JJE Caesar, Z Prohászka, TS Jokiranta, SM Lea, S Rodríguez, M Józsi
    J. Immunol., 2017-05-22;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3
    Authors: H He, AE Kuriyan, CW Su, M Mahabole, Y Zhang, YT Zhu, HW Flynn, JM Parel, SC Tseng
    Sci Rep, 2017-03-02;7(0):43736.
    Species: Human
    Sample Types: Whole Cells
  7. Three pentraxins C-reactive protein, serum amyloid p component and pentraxin 3 mediate complement activation using Collectin CL-P1
    Authors: Nobutaka Wakamiya
    Biochim. Biophys. Acta, 2016-11-15;0(0):.
  8. Factor H-related protein 5 interacts with pentraxin 3 and the extracellular matrix and modulates complement activation.
    Authors: Csincsi A, Kopp A, Zoldi M, Banlaki Z, Uzonyi B, Hebecker M, Caesar J, Pickering M, Daigo K, Hamakubo T, Lea S, Goicoechea de Jorge E, Jozsi M
    J Immunol, 2015-04-08;194(10):4963-73.
    Species: Human
    Sample Types: Protein
    Applications: Binding Assay
  9. The long pentraxin PTX3 promotes fibrocyte differentiation.
    Authors: Pilling D, Cox N, Vakil V, Verbeek J, Gomer R
    PLoS ONE, 2015-03-16;10(3):e0119709.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Role of pentraxin 3 in shaping arthritogenic alphaviral disease: from enhanced viral replication to immunomodulation.
    Authors: Foo, Suan-Sin, Chen, Weiqiang, Taylor, Adam, Sheng, Kuo-Chin, Yu, Xing, Teng, Terk-Shi, Reading, Patrick, Blanchard, Helen, Garlanda, Cecilia, Mantovani, Alberto, Ng, Lisa F P, Herrero, Lara J, Mahalingam, Suresh
    PLoS Pathog, 2015-02-19;11(2):e1004649.
    Species: Human
    Sample Types: Virus
    Applications: Bioassay
  11. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Atypical hemolytic uremic syndrome-associated variants and autoantibodies impair binding of factor h and factor h-related protein 1 to pentraxin 3.
    J. Immunol., 2012-07-11;189(4):1858-67.
    Species: Human
    Sample Types: Protein
    Applications: Binding Assay
  13. Pentraxin 3 (PTX3) expression in allergic asthmatic airways: role in airway smooth muscle migration and chemokine production.
    Authors: Zhang J, Shan L, Koussih L, Redhu NS, Halayko AJ, Chakir J, Gounni AS
    PLoS ONE, 2012-04-18;7(4):e34965.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding.
    Authors: Butler GS, Dean RA, Tam EM, Overall CM
    Mol. Cell. Biol., 2008-05-27;28(15):4896-914.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Pentraxin 3/TSG-14 Protein
By Balaji Mahender on 12/01/2017
Application: Binding assay/Protein-protein interaction