Recombinant Mouse IL-28B/IFN-lambda 3 Protein Summary
Product Specifications
Asp20-Val193, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1789-ML
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1789-ML/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-28B/IFN-lambda 3
Human IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and the type I IFN family (15 - 19% aa sequence identity) (1 - 3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon. The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta ) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory regions of the target genes. Mouse IL-28B cDNA encodes a 193 amino acid residue precursor protein with a putative 15 aa signal peptide. It shares 61%, 62% and 52% aa sequence identity with human IL-28A, human IL-28B and human IL-29, respectively.
- Vilcek, J. (2003) Nature Immunol. 4:8.
- Sheppard, P. et al. (2003) Nature Immunol. 4:63.
- Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.
Citations for Recombinant Mouse IL-28B/IFN-lambda 3 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Interferon inhibits the release of herpes simplex virus-1 from the axons of sensory neurons
Authors: Danastas, K;Guo, G;Merjane, J;Hong, N;Larsen, A;Miranda-Saksena, M;Cunningham, AL;
mBio
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Inhaled delivery of recombinant interferon-lambda restores allergic inflammation after development of asthma by controlling Th2- and Th17-cell-mediated immune responses
Authors: J Won, A Jo, CH Gil, S Kim, H Shin, H Jik Kim
International immunopharmacology, 2022-08-27;112(0):109180.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Intratumoral IL-28B Gene Delivery Elicits Antitumor Effects by Remodeling of the Tumor Microenvironment in H22-Bearing Mice
Authors: Z Li, J Wang, C Chen, Q He, X Xu, Z Da, B Wang, M Wang, X Gao, G Zhang, Q Gao, X Si, Y Luo, X Ma
Journal of Immunology Research, 2022-07-28;2022(0):1345971.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Mesenchymal stem cell-derived interleukin-28 drives the selection of apoptosis resistant bone metastatic prostate cancer
Authors: JJ McGuire, JS Frieling, CH Lo, T Li, A Muhammad, HR Lawrence, NJ Lawrence, LM Cook, CC Lynch
Nature Communications, 2021-02-01;12(1):723.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Hypoxia and therapeutic treatment of EV-A71 with an immune modulator TLR7 agonist in a new immunocompetent mouse model
Authors: AT Liou, CC Liao, SF Chou, YS Chang, CS Chang, C Shih
J. Biomed. Sci., 2019-11-11;26(1):93.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Type I and III IFNs Produced by Plasmacytoid Dendritic Cells in Response to a Member of the Flaviviridae Suppress Cellular Immune Responses
Authors: E Reid, N Juleff, M Windsor, S Gubbins, L Roberts, S Morgan, G Meyers, E Perez-Mart, E Tchilian, B Charleston, J Seago
J Immunol, 2016-04-06;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Control of hepatitis C virus replication in mouse liver-derived cells by MAVS-dependent production of type I and type III interferons.
Authors: g, Frentzen A, Gurlevik E, Yuan Q, Steinmann E, Ott M, Staeheli P, Schmid-Burgk J, Schmidt T, Hornung V, Kuehnel F, Pietschmann T
J Virol, 2015-01-21;89(7):3833-45.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
J. Virol., 2010-09-29;84(24):12703-12.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE).
Authors: Rynda A, Maddaloni M, Ochoa-Reparaz J, Callis G, Pascual DW
PLoS ONE, 2010-01-14;5(1):e8720.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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