Recombinant Mouse OX40 Ligand/TNFSF4 Protein Summary
Product Specifications
Gln49-Leu198, with an N-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1236-OX
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1236-OX/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: OX40 Ligand/TNFSF4
OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Murine OX40L cDNA encodes a 198 amino acid (aa) residue protein comprised of a 28 aa N-terminal cytoplasmic domain, a 20 aa transmembrane segment, and a 150 aa C-terminal extracellular domain (1). Human and murine OX40L share 46% sequence identity at the amino acid level (1). The OX40L is expressed on activated antigen presenting cells such as B cells, macrophages, dendritic cells, and on endothelial cells at the site of inflammation. The receptor for OX40L is OX40 (CD134) that is expressed predominantly on activated CD4+ T cells. Expression of OX40 is transient following engagement of T cell receptors (2). Ligation of OX40L by OX40 stimulates proliferation and differentiation of activated B cells, and increases immunoglobulin secretion (3, 4). The expression of OX40L on B cells is up-regulated by CD40 ligation (3). Engagement of the OX40-OX40L system has co-stimulatory effects on T cells by stimulating the production of cytokines by T helper cells and increasing the survival of memory T cells (2, 5). Blocking of the OX40-OX40L interaction in vitro inhibits co-stimulation resulting in decreased T cell proliferation and adhesion of T cells to endothelial cells. Inhibition of the OX40-OX40L interaction in disease models has beneficial effects in acute graft-versus-host disease, inflammatory bowel disease and decreases the development of collagen-induced arthritis and experimental leishmaniasis (6).
- Baum, P.R. et al. (1994) EMBO J. 13:3992.
- Gramaglia, I. et al. (1999) J. Immunol. 161:6510.
- Stuber, E. et al. (1995) Immunity 2:507.
- Malstrom, V. et al. (2001) J. Immunol. 166:6972.
- Maxwell, J.R. et al. (2000) J. Immunol. 164:107.
- Weinberg, A.D. (2002) Trends Immunol. 23:102.
Citations for Recombinant Mouse OX40 Ligand/TNFSF4 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity
Authors: P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung
Cell Rep, 2019-06-11;27(11):3117-3123.e5.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
OX40 Regulates Both Innate and Adaptive Immunity and Promotes Nonalcoholic Steatohepatitis
Authors: G Sun, H Jin, C Zhang, H Meng, X Zhao, D Wei, X Ou, Q Wang, S Li, T Wang, X Sun, W Shi, D Tian, K Liu, H Xu, Y Tian, X Li, W Guo, J Jia, Z Zhang, D Zhang
Cell Rep, 2018-12-26;25(13):3786-3799.e4.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Nr4a Receptors Regulate Development and Death of Labile Treg Precursors to Prevent Generation of Pathogenic Self-Reactive Cells
Authors: T Sekiya, S Hibino, K Saeki, M Kanamori, S Takaki, A Yoshimura
Cell Rep, 2018-08-07;24(6):1627-1638.e6.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Crucial role of OX40/OX40L signaling in a murine model of asthma
Authors: W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang
Mol Med Rep, 2018-01-18;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Guidance of super-enhancers in regulation of IL-9 induction and airway inflammation
Authors: X Xiao, Y Fan, J Li, X Zhang, X Lou, Y Dou, X Shi, P Lan, Y Xiao, L Minze, XC Li
J. Exp. Med., 2018-01-16;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Highly reduced binding to high and low affinity mouse Fc gamma receptors by L234A/L235A and N297A Fc mutations engineered into mouse IgG2a.
Authors: Arduin E, Arora S, Bamert P, Kuiper T, Popp S, Geisse S, Grau R, Calzascia T, Zenke G, Kovarik J
Mol Immunol, 2014-10-18;63(2):456-63.
Species: Mouse
Sample Types: Whole Cells
Applications: Binding Assay -
TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant.
Authors: Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y
Biochem. Biophys. Res. Commun., 2009-05-04;384(3):296-300.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
FAQs
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This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?
The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.
Reviews for Recombinant Mouse OX40 Ligand/TNFSF4 Protein
Average Rating: 5 (Based on 1 Review)
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