Recombinant Human TNF RII/TNFRSF1B (aa 24-206) Protein
Recombinant Human TNF RII/TNFRSF1B (aa 24-206) Protein Summary
Product Specifications
Pro24-Thr206
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1089-R2
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1089-R2/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: TNF RII/TNFRSF1B
TNF RI (also known as the p60 or p55 TNFR) and TNF RII (the p75 or p80 TNFR) are two distinct type I transmembrane glycoproteins that bind TNF with high-affinity. Both RI and RII are prototypic members of the TNF receptor superfamily and have been designated TNFRSF1A and TNFRSF1B, respectively. The extracellular domains of both receptors contain four conserved cysteine-rich domains while their intracellular domains are dissimilar, indicative of distinct signaling pathways. Human TNF RII cDNA encodes a 461 amino acid (aa) residue precursor protein with a 22 aa putative signal peptide, a 235 aa extracellular domain, a 20 aa transmembrane domain and a 174 aa cytoplasmic domain. Whereas all cell types studied express TNF RI, TNF RII expression is limited primarily to hematopoietic cells and cells of the immune system. Most of the biological functions of TNF are mediated via TNF RI. Soluble forms of both TNF RI and TNF RII, generated as a result of proteolytic cleavage of the extracellular domains, have been detected in vivo in various biological fluids. The soluble TNF receptors can bind TNF with high affinity and function as TNF antagonists (1 - 4).
- Chen G. and D.V. Goeddel (2002) Science 296:1634.
- MacEwan, D.J. (2002) Cell Signal. 14:477.
- Locksley, R.M. et al. (2001) Cell 104:487.
- Feldmann, M.and R.N. Maini (2001) Annu. Rev. Immunol. 19:163.
Citations for Recombinant Human TNF RII/TNFRSF1B (aa 24-206) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Inflammation-Driven Regulation of PD-L1 and PD-L2, and Their Cross-Interactions with Protective Soluble TNFalpha Receptors in Human Triple-Negative Breast Cancer
Authors: T Baram, N Oren, N Erlichman, T Meshel, A Ben-Baruch
Cancers, 2022-07-19;14(14):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay -
Characterization of the TNF and IL-1 systems in human brain and blood after ischemic stroke
Authors: BH Clausen, M Wirenfeldt, SS Høgedal, LH Frich, HH Nielsen, HD Schrøder, K Østergaard, B Finsen, BW Kristensen, KL Lambertsen
Acta Neuropathol Commun, 2020-06-05;8(1):81.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay -
Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1.
Authors: Steeland S, Puimege L, Vandenbroucke R, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C
J Biol Chem, 2014-12-23;290(7):4022-37.
Species: Human
Sample Types: Complex Sample Type
Applications: Enzyme Assay -
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Authors: Copeland S, Siddiqui J, Remick D
J. Immunol. Methods, 2004-01-01;284(1):99-106.
Applications: ELISA (Standard)
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Average Rating: 5 (Based on 1 Review)
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We used MAB726 as the solid phase antibody, 1089-R2 as the immunoassay standard and BAF225 as the detection antibody in a sandwich ELISA. Everything worked very well.
