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An reaction mixture containing CD39L2 and the kinase is added to each well of a 96-well microplate. |
A substrate mixture containing ATP and the acceptor substrate is then added to each well of the microplate to initiate the kinase reaction. |
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The Malachite Green Phosphate Detection Reagents are added to the wells. The reactions are terminated and a green color develops in proportion to the amount of inorganic phosphate released by the coupling phosphatase. The absorbance of the color at 620 nm is measured. |
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