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| The sample is added to a mixture of color-coded beads, pre-coated with analyte-specific capture antibodies. The antibodies bind to the analytes of interest. | Biotinylated detection antibodies specific to the analytes of interest are added and form an antibody-antigen sandwich. Phycoerythrin (PE)-conjugated streptavidin is added. It binds to the biotinylated detection antibodies. |
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| Beads are read on a dual-laser flow-based detection instrument, such as the Luminex 200™ or FlexMap® analyzer. One laser classifies the bead and determines the analyte that is being detected. The second laser determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. |
In addition to flow-based analyzers, the xMAP INTELLIFLEX® is an advanced multiplexing instrument that offers automation capabilities. It boasts a compact design with a reduced footprint and a wide dynamic range, providing powerful performance. The instrument enables the simultaneous detection of up to 500 analytes in a single sample volume and offers high sensitivity and accuracy. |
Find Your Luminex® Answers
R&D Systems’ Luminex assays have the largest, most flexible Luminex analyte available. With our menu of off-the-shelf fixed panels, high-performance configurable panels, and completely custom panels, we have options to best fit your needs, ensuring you get the most data from your samples with results you can trust.
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Luminex®, Luminex 200™, Luminex xMAP INTELLIFLEX®, and FlexMap® are trademarks of Luminex Corporation.