Viral wnvNS1 Protein Antibody

Catalog #: MAB290721 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB290721-100
MAB290721-SP
Viral wnvNS1 Protein ELISA Standard Curve.
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Viral wnvNS1 Protein Antibody Summary

Species Reactivity
Viral
Specificity
Detects recombinant Viral wnvNS1 protein in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 991502
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with recombinant Viral wnvNS1 Protein
Asp1-Ala352, Asp1-Ala352
Accession # NP_776015
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral wnvNS1 Protein Monoclonal Antibody (Catalog # MAB290722).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Viral wnvNS1 Protein ELISA Standard Curve. Recombinant Viral wnvNS1 Protein protein was serially diluted 2-fold and captured by Mouse Anti-Viral wnvNS1 Protein Monoclonal Antibody (Catalog # MAB290722) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral wnvNS1 Protein Monoclonal Antibody (Catalog # MAB290721) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: wnvNS1 Protein

West Nile Virus, or WNV, is a member of the Flaviviridae family that includes other human pathogens such as yellow fever (YF), dengue (DEN), tick-borne encephalitis (TBE), Japanese encephalitis (JEV), and Murray Valley encephalitis (MVE) viruses. West Nile virus (WNV) is a single-stranded RNA mosquito-transmitted virus that causes epidemics of encephalitis and viscerotropic disease. The WNV nonstructural protein NS1 is a 48-kDa highly conserved secreted glycoprotein that does not package with the virion. Even when absent from the virion, immunization with NS1 typically elicits a protective immune response.

Long Name
West Nile Virus Non-structural Protein NS1
Entrez Gene IDs
912267 (Viral)
Alternate Names
wnvNS1 Protein

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