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Viral MIP-I Antibody

Catalog #: MAB799 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB799
MAB799-SP
Chemotaxis Induced by MIP‑I and Neutralization by Viral MIP‑I Antibody.
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Viral MIP-I Antibody Summary

Species Reactivity
Viral
Specificity
Detects viral MIP-I in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant cytomegalovirus UL146, recombinant human (rh) CCL3, rhCCL22, recombinant mouse (rh) CCL3 or rmCCL22 is observed.
Source
Monoclonal Mouse IgG2B Clone # 84420
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human herpes virus-8 MIP-I
Ala25-Ala95
Accession # YP_001129366
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Viral MIP-I (Catalog # 600-VA)
Neutralization
Measured by its ability to neutralize MIP‑I-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 0.02 µg/mL Recombinant Viral MIP‑I.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by MIP‑I and Neutralization by Viral MIP‑I Antibody. View Larger

Chemotaxis Induced by MIP‑I and Neutralization by Viral MIP‑I Antibody. Recombinant Viral MIP-I (Catalog # 600-VA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Viral MIP-I (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Viral MIP-I Monoclonal Antibody (Catalog # MAB799). The ND50 is typically 0.1-0.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIP-I

Human herpesvirus-8 (HHV‑8)/Kaposi’s sarcoma-associated herpesvirus (KSHV) is a gamma  herpesvirus with homology to herpesvirus Saimiri and Epstein-Barr virus. HHV‑8 is etiologically linked to Kaposi’s sarcoma and a B-cell lymphoma known as primary effusion lymphoma. HHV‑8 has been shown to encode a variety of immunomodulatory proteins which were apparently pirated from cellular genes by the virus. Three chemokine-like proteins, vMIP-I, vMIP-II and vMIP-III have been found to be encoded within the HHV‑8 genome.

Viral MIP-I (also termed vMIP-1 alpha ) cDNA encodes a 95 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide that is cleaved to yield a 71 aa residue mature protein. Among human chemokines, vMIP-I is most closely related to MIP-1 alpha, sharing approximately 38% amino acid sequence identity. At the amino acid sequence level, vMIP-I and vMIP-II also share 48% identity. vMIP-I and vMIP-II are more closely related to one another phylogenetically than to other human chemokines, suggesting that they may have arisen by gene duplication within the virus rather than by two independent gene aquisitions. Both vMIP-I and vMIP-II have been shown to partially block HIV infection of peripheral blood mononuclear cells. vMIP-I and vMIP-II have also been found to be highly angiogenic in the chorioallantoic assay, suggesting that they may be partially responsible for the marked vascularity seen in KSHV-associated tumors.

References
  1. Moore, P.S. et al. (1996) Science 274:5293.
  2. Boshoff, C. et al. (1997) Science 278:290.
Long Name
Macrophage Inflammatory Protein I
Alternate Names
MIPI; MIP-I

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