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VasoTACS In Situ Apoptosis Detection Kit

Catalog #: 4826-30-K
5 Citations Datasheet / COA / SDS
In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded vascular cells and tissues
Catalog # Availability Size / Price Qty
4826-30-K
VasoTACS In Situ Apoptosis Detection Kit_Rat Artery Tissue
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Product Details
Citations (5)
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VasoTACS In Situ Apoptosis Detection Kit Summary

Provides an effective in situ method for identifying apoptosis in vascular samples using an optimized TUNEL based assay.
 

Key Benefits

• Performance tested on vascular tissues.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagents.
• Helps resolve unique problems encountered when detecting apoptotic vascular cells.

Why Use the VasoTACS In Situ Apoptosis Detection Kit?

This kit allows the user to successfully label apoptotic cells, including endothelial and smooth muscle cells, throughout the vascular system. Similar to the CardioTACS Kit, this kit is also based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label detection system with Red Counterstain C. Specifically, VasoTACS™ has been tested and optimized to help resolve the unique problems encountered when detecting apoptotic vascular cells.

Kit Contents

• Proteinase K
• Streptavidin-HRP
• TACS-Blue Label
• Blue Strep-HRP Diluent
• TACS-Nuclease
• TACS-Nuclease Buffer
• Red Counterstain C
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Manganese Cation
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

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COA

Scientific Data

N/A VasoTACS In Situ Apoptosis Detection Kit_Rat Artery Tissue View Larger

Visualization of Apoptotic Cells in Vascular Tissue using the VasoTACS In Situ Apoptosis Detection Kit. TIssue from the small artery of rat was formalin-fixed and paraffin-embedded following the initiation of drug-induced apoptosis. The tissue was processed according to the protocol in the VasoTACS Kit (Catalog # 4826-30-K) and visualized by light microscopy. Brown stained nuclei following counterstaining with Red Counterstain C indicated apoptotic cells. Data was provided courtesy of Dr. Jun Zhang, FDA.

Citations for VasoTACS In Situ Apoptosis Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Kallistatin limits abdominal aortic aneurysm by attenuating generation of reactive oxygen species and apoptosis
    Authors: SM Krishna, J Li, Y Wang, CS Moran, A Trollope, P Huynh, R Jose, E Biros, J Ma, J Golledge
    Scientific Reports, 2021-08-31;11(1):17451.  2021-08-31
  2. Endothelial lipid phosphate phosphatase-3 deficiency that disrupts the endothelial barrier function is a modifier of cardiovascular development.
    Authors: Chatterjee I, Baruah J, Lurie E, Wary K
    Cardiovasc Res, 2016-04-28;111(1):105-18.  2016-04-28
  3. Dexamethasone induces apoptosis in pulmonary arterial smooth muscle cells.
    Authors: Price L, Shao D, Meng C, Perros F, Garfield B, Zhu J, Montani D, Dorfmuller P, Humbert M, Adcock I, Wort S
    Respir Res, 2015-09-18;16(0):114.  2015-09-18
  4. Critical role for GATA3 in mediating Tie2 expression and function in large vessel endothelial cells.
    Authors: Song H, Suehiro J, Kanki Y, Kawai Y, Inoue K, Daida H, Yano K, Ohhashi T, Oettgen P, Aird WC, Kodama T, Minami T
    J. Biol. Chem., 2009-08-12;284(42):29109-24.  2009-08-12
  5. The Down syndrome critical region gene 1 short variant promoters direct vascular bed-specific gene expression during inflammation in mice.
    Authors: Minami T, Yano K, Miura M, Kobayashi M, Suehiro J, Reid PC, Hamakubo T, Ryeom S, Aird WC, Kodama T
    J. Clin. Invest., 2009-07-13;119(8):2257-70.  2009-07-13

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