Ubiquitin-Propargylamide Protein Summary
Product Specifications
Met1 - Gly75
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
U-214
| Formulation | Lyophilized from a solution containing Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 2 mg/ml in DMSO. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Ubiquitin
Ubiquitin-Propargylamine (Ub-PA) is a potent, irreversible and specific inhibitor of C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs), and other deubiquitinases including the Otubains (OTU’s). When mixed together, the reactive site cysteine of deubiquitinases and the C-terminal alkyne functional group of Ub-PA rapidly form a covalent bond (vinyl thioether) that is resistant to denaturing conditions. This product is useful for inhibiting the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation.
Citations for Ubiquitin-Propargylamide Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Sde Proteins Coordinate Ubiquitin Utilization and Phosphoribosylation to Promote Establishment and Maintenance of the Legionella Replication Vacuole
Authors: Isberg, R;Kotewicz, K;Zheng, M;Kim, S;Tai, A;
Research square
Species: N/A
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR) -
Structural basis for the dual catalytic activity of the Legionella pneumophila ovarian-tumor (OTU) domain deubiquitinase LotA
Authors: J Luo, X Ruan, Z Huang, Z Li, L Ye, Y Wu, X Zhen, S Ouyang
The Journal of Biological Chemistry, 2022-08-22;0(0):102414.
Species: Bacteria - Legionella pneumophila
Sample Types: Recombinant Protein
Applications: Bioassay
FAQs
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View all Proteins and Enzyme FAQsReviews for Ubiquitin-Propargylamide Protein
Average Rating: 4 (Based on 1 Review)
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Reason for Rating: USP15 is well labeled with this probe.
293T cell lysates （40μg）were incubated with this probe for 15 min at 37℃。
