Trypsin 0.05% – EDTA 0.53 mM (1X)
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Trypsin 0.05% – EDTA 0.53 mM (1X) Summary
Contains 0.5 gm/L of trypsin (1:250) and 0.2 gm/L EDTA•4Na in Hanks’ Balanced Salt Solution without calcium and magnesium salts.Why Use Trypsin for Cell Culture?
Trypsin 0.05% is the trypsin solution of choice for more sensitive cell lines. EDTA (Ethylenediaminetetraacetic acid), a chelating agent that neutralizes calcium and magnesium ions, is added to increase the enzymatic activity on the cells and to facilitate release of individual cells from the culture vessel. Calcium and magnesium ions enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. Trypsin is the most commonly used trypsin-based dissociation reagents for cell isolation, allowing for a simple and reliable dissociation process in cell culture. Each product is carefully manufactured to minimize loss of enzyme activity from denaturation and autodegradation, and quality controlled to ensure consistency and reliability.
Specifications
Limitations
For research use only. Not for diagnostic use.
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