TACS 2 TdT-DAB In Situ Apoptosis Detection Kit

In situ detection of apoptosis by TUNEL in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
Catalog # Availability Size / Price Qty
4810-30-K
Product Details
Citations (99)
FAQs
Reviews (1)

TACS 2 TdT-DAB In Situ Apoptosis Detection Kit Summary

Provides a unique cation optimization system to enhance the in situ detection of apoptosis in a TUNEL based assay.
 

Key Benefits

• TdT based in situ labeling.
• Cation optimization for high signal and low background.
• Complete kit providing DAB and Methyl Green Counterstain.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagent.
• Includes TACS-Nuclease control reagents.

Why Use the TACS 2 TdT-DAB In Situ Apoptosis Detection Kit?

The TACS 2 TdT DAB Kit utilizes a cation optimization system to enhance the in situ detection of apoptosis in a TUNEL based assay within fixed frozen, paraffin embedded, or plastic embedded cells and tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA is performed by a highly purified form of the TdT enzyme in a non-toxic labeling buffer (sodium cacodylate free). Biotin labeling is detected using streptavidin-horseradish peroxidase, and colorimetric substrates diaminobenzidine (DAB). Samples are counterstained with Methyl Green offering a nice contrast when visualized by light microscopy.

This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffers, counterstain, and TACS-Nuclease reagents for generating positive controls with your own samples.

Product Specifications

  • In situ detection of apoptosis by TUNEL-based assay in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Light microscopy.

Kit Contents

• Proteinase K
• Diaminobenzidine
• DAB Enhancer
• TACS-Nuclease
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Cobalt Cation
• 50x Magnesium Cation
• 50x Manganese Cation
• Streptavidin-HRP
• TACS-Nuclease™ Buffer
• Methyl Green 1%
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

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Citations for TACS 2 TdT-DAB In Situ Apoptosis Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

99 Citations: Showing 1 - 10
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  1. Single-cell transcriptomics identifies the differentiation trajectory from inflammatory monocytes to pro-resolving macrophages in a mouse skin allergy model
    Authors: Miyake, K;Ito, J;Takahashi, K;Nakabayashi, J;Brombacher, F;Shichino, S;Yoshikawa, S;Miyake, S;Karasuyama, H;
    Nature communications  2024-02-23
  2. O-GlcNAc transferase promotes glioblastoma by modulating genes responsible for cell survival, invasion, and inflammation
    Authors: Sheikh, MA;Alawathugoda, TT;Vyas, G;Emerald, BS;Ansari, SA;
    The Journal of biological chemistry  2023-09-07
  3. Inhibiting miR-195-5p Induces Proliferation of Human Corneal Endothelial Cells
    Authors: Parekh, M;Ramos, T;Ferrari, S;Ahmad, S;
    International journal of molecular sciences  2023-07-15
  4. Structural and functional integrity of endocrine pancreas post administration of Karwinskia humboldtiana fruit to Wistar rats: a possible therapeutic application for cancer of exocrine origin
    Authors: JC Segoviano-, N Esparza-Ro, K Carcano-Di, RN Diaz-Perez, JP Palma-Nico, R Hernandez-, J Garcia-Jua
    Histology and histopathology, 2023-03-02;0(0):18603.  2023-03-02
  5. Sirolimus-Embedded Silk Microneedle Wrap to Prevent Neointimal Hyperplasia in Vein Graft Model
    Authors: JH Kim, EH Jang, JY Ryu, J Lee, JH Kim, W Ryu, YN Youn
    International Journal of Molecular Sciences, 2023-02-07;24(4):.  2023-02-07
  6. Developing 3D Organoid Raft Cultures from Patient-Derived Xenografts as Rapid Models to Screen Efficacy of Experimental Therapeutics
    Authors: P Bajpai, NS Banerjee, DW Moore, HG Kim, F Afaq, CM Contreras, MJ Heslin, VB Reddy, S Peter, S Varambally, S Al Diffalh, U Manne
    International Journal of Molecular Sciences, 2022-11-19;23(22):.  2022-11-19
  7. Developmental basis of evolutionary lung loss in plethodontid salamanders
    Authors: ZR Lewis, R Kerney, J Hanken
    Science Advances, 2022-08-17;8(33):eabo6108.  2022-08-17
  8. EGFR ligand shifts the role of EGFR from oncogene to tumour suppressor in EGFR-amplified glioblastoma by suppressing invasion through BIN3 upregulation
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    Nature Cell Biology, 2022-08-01;24(8):1291-1305.  2022-08-01
  9. Saireito, a Japanese herbal medicine, alleviates leaky gut associated with antibiotic-induced dysbiosis in mice
    Authors: S Ozaka, A Sonoda, S Ariki, M Minata, N Kamiyama, S Hidano, N Sachi, K Ito, Y Kudo, A Dewayani, T Chalalai, T Ozaki, Y Soga, C Fukuda, K Mizukami, S Ishizawa, M Nishiyama, N Fujitsuka, S Mogami, K Kubota, K Murakami, T Kobayashi
    PLoS ONE, 2022-06-15;17(6):e0269698.  2022-06-15
  10. Implication of COPB2 Expression on Cutaneous Squamous Cell Carcinoma Pathogenesis
    Authors: T Chen, KY Kim, Y Oh, HC Jeung, KY Chung, MR Roh, X Zhang
    Cancers, 2022-04-18;14(8):.  2022-04-18
  11. Peroxiredoxin 3 deficiency induces cardiac hypertrophy and dysfunction by impaired mitochondrial quality control
    Authors: SK Sonn, EJ Song, S Seo, YY Kim, JH Um, FJ Yeo, DS Lee, S Jeon, MN Lee, J Jin, HY Kweon, TK Kim, S Kim, SH Moon, SG Rhee, J Chung, J Yang, J Han, EY Choi, SB Lee, J Yun, GT Oh
    Redox Biology, 2022-02-28;51(0):102275.  2022-02-28
  12. MSK-Mediated Phosphorylation of Histone H3 Ser28 Couples MAPK Signalling with Early Gene Induction and Cardiac Hypertrophy
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    Cells, 2022-02-09;11(4):.  2022-02-09
  13. Methodology and Neuromarkers for Cetaceans' Brains
    Authors: S Sacchini, P Herráez, M Arbelo, A Espinosa d, E Sierra, M Rivero, C Bombardi, A Fernández
    Veterinary sciences, 2022-01-21;9(2):.  2022-01-21
  14. Reduced hyaluronan cross-linking induces breast cancer malignancy in a CAF-dependent manner
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    Cell Death & Disease, 2021-06-07;12(6):586.  2021-06-07
  15. CSF1R-dependent macrophages control postnatal somatic growth and organ maturation
    Authors: S Keshvari, M Caruso, N Teakle, L Batoon, A Sehgal, OL Patkar, M Ferrari-Ce, CE Snell, C Chen, A Stevenson, FM Davis, SJ Bush, C Pridans, KM Summers, AR Pettit, KM Irvine, DA Hume
    PloS Genetics, 2021-06-03;17(6):e1009605.  2021-06-03
  16. Islet Dysfunction in a Novel Transgenic Model of T Cell Insulitis
    Authors: E Esakov, N Nandedkar-, AG Al-Dieri, H Hafner, B Gregg, MF McInerney
    Biomolecules, 2021-04-09;11(4):.  2021-04-09
  17. SIRT6?specific inhibitor OSS?128167 exacerbates diabetic cardiomyopathy by aggravating inflammation and oxidative stress
    Authors: Y Huang, J Zhang, D Xu, Y Peng, Y Jin, L Zhang
    Molecular Medicine Reports, 2021-03-24;23(5):.  2021-03-24
  18. MTH1 and OGG1 maintain a low level of 8-oxoguanine in Alzheimer's brain, and prevent the progression of Alzheimer's pathogenesis
    Authors: S Oka, J Leon, K Sakumi, N Abolhassan, Z Sheng, D Tsuchimoto, FM LaFerla, Y Nakabeppu
    Scientific Reports, 2021-03-23;11(1):5819.  2021-03-23
  19. Remote effects and biodistribution of pulmonary instilled silver nanoparticles in mice
    Authors: Z Ferdous, S Al-Salam, P Yuvaraju, BH Ali, A Nemmar
    NanoImpact, 2021-03-13;22(0):100310.  2021-03-13
  20. Preferable Transplant Site for Hepatocyte Transplantation in a Rat Model
    Authors: H Ogasawara, A Inagaki, I Fathi, T Imura, H Yamana, Y Saitoh, M Matsumura, K Fukuoka, S Miyagi, Y Nakamura, K Ohashi, M Unno, T Kamei, M Goto
    Cell Transplantation, 2021-01-01;30(0):9636897211040.  2021-01-01
  21. Periadventitial Delivery of Simvastatin-Loaded Microparticles Attenuate Venous Neointimal Hyperplasia Associated With Arteriovenous Fistula
    Authors: C Zhao, ST Zuckerman, C Cai, S Kilari, A Singh, M Simeon, HA von Recum, JN Korley, S Misra
    Journal of the American Heart Association, 2020-12-05;9(24):e018418.  2020-12-05
  22. Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway
    Authors: SJ Lee, J Park, DJ Lee, K Otsu, P Kim, S Mizuno, MJ Lee, HY Kim, H Harada, S Takahashi, SJ Kim, HS Jung
    Cell Death Differ, 2020-11-20;0(0):.  2020-11-20
  23. Inhibition of NLRP3 inflammasome by MCC950 improves the metabolic outcome of islet transplantation by suppressing IL-1&beta and islet cellular death
    Authors: T Matsuoka, G Yoshimatsu, N Sakata, R Kawakami, T Tanaka, T Yamada, Y Yoshida, S Hasegawa, S Kodama
    Sci Rep, 2020-10-21;10(1):17920.  2020-10-21
  24. Spred2-deficiency enhances the proliferation of lung epithelial cells and alleviates pulmonary fibrosis induced by bleomycin
    Authors: A Kawara, R Mizuta, M Fujisawa, T Ito, C Li, K Nakamura, C Sun, M Kuwabara, M Kitabatake, T Yoshimura, A Matsukawa
    Sci Rep, 2020-10-05;10(1):16490.  2020-10-05
  25. NF-kappaB inhibitor with Temozolomide results in significant apoptosis in glioblastoma via the NF-kappaB(p65) and actin cytoskeleton regulatory pathways
    Authors: NG Avci, S Ebrahimzad, YM Akay, Y Esquenazi, N Tandon, JJ Zhu, M Akay
    Sci Rep, 2020-08-07;10(1):13352.  2020-08-07
  26. Compensatory combination of romidepsin with gemcitabine and cisplatin to effectively and safely control urothelial carcinoma
    Authors: P Pattarawat, T Hong, S Wallace, Y Hu, R Donnell, TH Wang, CL Tsai, J Wang, HR Wang
    Br. J. Cancer, 2020-05-11;0(0):.  2020-05-11
  27. Enhanced Biocompatibility of Multi-Layered, 3D Bio-Printed Artificial Vessels Composed of Autologous Mesenchymal Stem Cells
    Authors: EH Jang, JH Kim, JH Lee, DH Kim, YN Youn
    Polymers (Basel), 2020-03-02;12(3):.  2020-03-02
  28. Endothelial autophagy deficiency induces IL6 - dependent endothelial mesenchymal transition and organ fibrosis
    Authors: Y Takagaki, SM Lee, Z Dongqing, M Kitada, K Kanasaki, D Koya
    Autophagy, 2020-01-22;0(0):1-10.  2020-01-22
  29. Targeting alkaline ceramidase 3 alleviates the severity of nonalcoholic steatohepatitis by reducing oxidative stress
    Authors: K Wang, C Li, X Lin, H Sun, R Xu, Q Li, Y Wei, Y Li, J Qian, C Liu, Q Zhang, S Yu, Z Cui, X Huang, B Zhu, J Zhou, C Mao
    Cell Death Dis, 2020-01-16;11(1):28.  2020-01-16
  30. Ginsenoside CK induces apoptosis and suppresses proliferation and invasion of human osteosarcoma cells through the PI3K/mTOR/p70S6K1 pathway
    Authors: K Chen, J Jiao, J Xue, T Chen, Y Hou, Y Jiang, L Qian, Y Wang, Z Ma, Z Liang, B Sun, Q Ren
    Oncol. Rep., 2020-01-13;43(3):886-896.  2020-01-13
  31. Inhibitor of DNA-Binding Protein 4 Suppresses Cancer Metastasis through the Regulation of Epithelial Mesenchymal Transition in Lung Adenocarcinoma
    Authors: CC Wang, YL Hsu, CJ Chang, CJ Wang, TH Hsiao, SH Pan
    Cancers (Basel), 2019-12-14;11(12):.  2019-12-14
  32. Attenuation of diabetic cardiomyopathy by relying on kirenol to suppress inflammation in a diabetic rat model
    Authors: B Wu, XY Huang, L Li, XH Fan, PC Li, CQ Huang, J Xiao, R Gui, S Wang
    J. Cell. Mol. Med., 2019-09-29;0(0):.  2019-09-29
  33. Triptolide sensitizes cisplatin-resistant human epithelial ovarian cancer by inhibiting the phosphorylation of AKT
    Authors: G Huang, H Hu, Y Zhang, Y Zhu, J Liu, B Tan, T Chen
    J Cancer, 2019-06-02;10(13):3012-3020.  2019-06-02
  34. VEGF-A from Granuloma Macrophages Regulates Granulomatous Inflammation by a Non-angiogenic Pathway during Mycobacterial Infection
    Authors: JS Harding, M Herbath, Y Chen, A Rayasam, A Ritter, B Csoka, G Hasko, IP Michael, Z Fabry, A Nagy, M Sandor
    Cell Rep, 2019-05-14;27(7):2119-2131.e6.  2019-05-14
  35. 8-Oxoguanine accumulation in aged female brain impairs neurogenesis in the dentate gyrus and major island of Calleja, causing sexually dimorphic phenotypes
    Authors: N Haruyama, K Sakumi, A Katogi, D Tsuchimoto, G De Luca, M Bignami, Y Nakabeppu
    Prog. Neurobiol., 2019-04-23;0(0):.  2019-04-23
  36. A Simple and Useful Predictive Assay for Evaluating the Quality of Isolated Hepatocytes for Hepatocyte Transplantation
    Authors: M Matsumura, T Imura, A Inagaki, H Ogasawara, K Fukuoka, I Fathi, S Miyagi, K Ohashi, M Unno, T Kamei, S Satomi, M Goto
    Sci Rep, 2019-04-16;9(1):6166.  2019-04-16
  37. Concomitant Inhibition of Cytoprotective Autophagy Augments the Efficacy of Withaferin A in Hepatocellular Carcinoma
    Authors: S Siddharth, N Muniraj, NK Saxena, D Sharma
    Cancers (Basel), 2019-03-30;11(4):.  2019-03-30
  38. Epithelial ovarian cancer: feasibility of image-guided intratumoral radiofrequency hyperthermia-enhanced direct gene therapy
    Authors: G Jin, Y Li, F Zhang, P Li, L Zhao, Y Zhou, H Ji, S Pietrini, B Zhai, X Yang
    Am J Cancer Res, 2019-02-01;9(2):378-389.  2019-02-01
  39. Impaired LXR? Phosphorylation Attenuates Progression of Fatty Liver Disease
    Authors: N Becares, MC Gage, M Voisin, E Shrestha, L Martin-Gut, N Liang, R Louie, B Pourcet, OM Pello, TV Luong, S Goñi, C Pichardo-A, H Røberg-Lar, V Diaz-Zucca, KR Steffensen, A O'Brien, MJ Garabedian, K Rombouts, E Treuter, I Pineda-Tor
    Cell Rep, 2019-01-22;26(4):984-995.e6.  2019-01-22
  40. Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon
    Authors: S Gurung, N Reuter, A Preno, J Dubaut, H Nadeau, K Hyatt, K Singleton, A Martin, WT Parks, JF Papin, DA Myers
    PLoS Pathog., 2019-01-18;15(1):e1007507.  2019-01-18
  41. MiR-30c/PGC-1? protects against diabetic cardiomyopathy via PPAR?
    Authors: Z Yin, Y Zhao, M He, H Li, J Fan, X Nie, M Yan, C Chen, DW Wang
    Cardiovasc Diabetol, 2019-01-11;18(1):7.  2019-01-11
  42. Schwannoma gene therapy by adeno-associated virus delivery of the pore-forming protein Gasdermin-D
    Authors: SG Ahmed, A Ahmed, M Doha, GJ Brenner
    Cancer Gene Ther., 2019-01-09;0(0):.  2019-01-09
  43. Human neural stem cell-derived neuron/astrocyte co-cultures respond to La Crosse virus infection with proinflammatory cytokines and chemokines
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    J Neuroinflammation, 2018-11-15;15(1):315.  2018-11-15
  44. Role of SIRT2 in Regulation of Stemness of Cancer Stem-Like Cells in Renal Cell Carcinoma
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  56. Regulation of Sirtuin 3-Mediated Deacetylation of Cyclophilin D Attenuated Cognitive Dysfunction Induced by Sepsis-Associated Encephalopathy in Mice.
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    Mol. Cancer Ther, 2017-02-21;0(0):.  2017-02-21
  58. PERK Is a Haploinsufficient Tumor Suppressor: Gene Dose Determines Tumor-Suppressive Versus Tumor Promoting Properties of PERK in Melanoma
    PLoS Genet, 2016-12-15;12(12):e1006518.  2016-12-15
  59. Differential cell-matrix responses in hypoxia-stimulated aortic versus mitral valves.
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  60. Smpd3 Expression in both Chondrocytes and Osteoblasts Is Required for Normal Endochondral Bone Development.
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    Mol Cell Biol, 2016-08-12;36(17):2282-99.  2016-08-12
  61. Chromatin assembly factor 1, subunit A (P150) facilitates cell proliferation in human hepatocellular carcinoma
    Onco Targets Ther, 2016-07-01;9(0):4023-35.  2016-07-01
  62. TLR4 Deficiency Impairs Oligodendrocyte Formation in the Injured Spinal Cord.
    Authors: Church J, Kigerl K, Lerch J, Popovich P, McTigue D
    J Neurosci, 2016-06-08;36(23):6352-64.  2016-06-08
  63. mTORC1-Driven Tumor Cells Are Highly Sensitive to Therapeutic Targeting by Antagonists of Oxidative Stress.
    Authors: Li J, Shin S, Sun Y, Yoon S, Li C, Zhang E, Yu J, Zhang J, Blenis J
    Cancer Res, 2016-05-17;76(16):4816-27.  2016-05-17
  64. Uterine natural killer cell partnerships in early mouse decidua basalis.
    Authors: Felker A, Croy B
    J Leukoc Biol, 2016-03-21;100(4):645-655.  2016-03-21
  65. Transient increase of fractional anisotropy in reversible vasogenic edema.
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  66. Targeted Deletion of p53 in Lgr5-Expressing Intestinal Stem Cells Promotes Colon Tumorigenesis in a Preclinical Model of Colitis-Associated Cancer.
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    Cancer Res, 2015-12-02;75(24):5392-7.  2015-12-02
  67. Granzyme B Deficiency Protects against Angiotensin II-Induced Cardiac Fibrosis.
    Authors: Shen Y, Cheng F, Sharma M, Merkulova Y, Raithatha S, Parkinson L, Zhao H, Westendorf K, Bohunek L, Bozin T, Hsu I, Ang L, Williams S, Bleackley R, Eriksson J, Seidman M, McManus B, Granville D
    Am J Pathol, 2015-11-25;186(1):87-100.  2015-11-25
  68. Acceleration of diabetic wound healing using a novel protease-anti-protease combination therapy.
    Authors: Gao M, Nguyen T, Suckow M, Wolter W, Gooyit M, Mobashery S, Chang M
    Proc Natl Acad Sci U S A, 2015-11-23;112(49):15226-31.  2015-11-23
  69. Folate Receptor-Targeted Polymeric Micellar Nanocarriers for Delivery of Orlistat as a Repurposed Drug against Triple-Negative Breast Cancer.
    Authors: Paulmurugan R, Bhethanabotla R, Mishra K, Devulapally R, Foygel K, Sekar T, Ananta J, Massoud T, Joy A
    Mol Cancer Ther, 2015-11-09;15(2):221-31.  2015-11-09
  70. Secreted Frizzled-Related Protein 3 (SFRP3) Is Required for Tumorigenesis of PAX3-FOXO1-Positive Alveolar Rhabdomyosarcoma.
    Authors: Kephart J, Tiller R, Crose L, Slemmons K, Chen P, Hinson A, Bentley R, Chi J, Linardic C
    Clin Cancer Res, 2015-06-12;21(21):4868-80.  2015-06-12
  71. Distinct Immune Responses in Resistant and Susceptible Strains of Mice during Neurovirulent Alphavirus Encephalomyelitis.
    Authors: Kulcsar K, Baxter V, Abraham R, Nelson A, Griffin D
    J Virol, 2015-06-03;89(16):8280-91.  2015-06-03
  72. The endogenous cell-fate factor dachshund restrains prostate epithelial cell migration via repression of cytokine secretion via a cxcl signaling module.
    Authors: Chen K, Wu K, Jiao X, Wang L, Ju X, Wang M, Di Sante G, Xu S, Wang Q, Li K, Sun X, Xu C, Li Z, Casimiro M, Ertel A, Addya S, McCue P, Lisanti M, Wang C, Davis R, Mardon G, Pestell R
    Cancer Res, 2015-03-13;75(10):1992-2004.  2015-03-13
  73. Neonatal uterine and vaginal cell proliferation and adenogenesis are independent of estrogen receptor 1 (ESR1) in the mouse.
    Authors: Nanjappa M, Medrano T, March A, Cooke P
    Biol Reprod, 2015-02-04;92(3):78.  2015-02-04
  74. FBXW7 acts as an independent prognostic marker and inhibits tumor growth in human osteosarcoma.
    Authors: Li, Zhanchun, Xiao, Jie, Hu, Kongzu, Wang, Gang, Li, Maoqiang, Zhang, Jidong, Cheng, Guangqi
    Int J Mol Sci, 2015-01-22;16(2):2294-306.  2015-01-22
  75. Synthetic lethality of combined glutaminase and Hsp90 inhibition in mTORC1-driven tumor cells.
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TdT In Situ Apoptosis Detection Kit - DAB
By Anonymous on 10/11/2016