TACS XTT Cell Proliferation Assay
TACS XTT Cell Proliferation Assay Summary
Provides a sensitive kit for the measurement of cell proliferation based upon the reduction of the tetrazolium salt, 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide.Key Benefits
• Allows measurement of cell proliferation, cell viability or cytotoxicity.
• Rapid determination in microplates (no solubilization step as in an MTT assay).
• Reproducible and sensitive results.
• No radioactivity.
• Ideal for high throughput assays (no washing or other steps that can cause cell loss and variability).
Why Use the TACS XTT Cell Proliferation Assay?
The XTT Cell Proliferation Assay (XTT-CPA) is a sensitive kit for the measurement of cell proliferation. Cleavage of the tetrazolium salt to formazan occurs via the succinate-tetrazolium reductase system in the mitochondria of metabolically active cells.
Kit Contents
• XTT Reagent
• XTT Activator
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Scientific Data
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Relative Cell Number Measured using the TACS XTT Cell Proliferation/Viability Assay. Murine WEHI cells were counted and serial diluted in a microplate using 100 µL of the listed densities. Cells were incubated with XTT for 6 hours at 27 °C and absorbance was read using an ELISA plate reader.
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RAW 264.7 cell line cultured with DMEM + fetal bovine serum 10%. Different cell quantities were seeded in 96 well plates (each quantity per triplicate). Incubation with XTT solution was 4 h. Data shows average + SEM.
