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Synthetic Human Ubiquitin Vinyl Sulfone Protein, CF

Catalog #: U-202B
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
U-202B-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (10)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Synthetic Human Ubiquitin Vinyl Sulfone Protein, CF Summary

Product Specifications

Purity
>95%, by HPLC.
Activity
Recombinant Human Ubiquitin-Vinyl Sulfone added to in vitro assays will inhibit deubiquitinating enzymes. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin-Vinyl Sulfone concentration of 1-5 μM.
Source
Chemically Synthesized Ubiquitin protein
Met1 - Gly75
Accession #
P0CG47.1
Predicted Molecular Mass
8.6 kDa

Product Datasheets

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U-202B

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

U-202B

Formulation Supplied as a solution in MES and DMSO.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7). Ubiquitin-Vinyl Sulfone is a potent, irreversible and specific inhibitor of Ubiquitin C-terminal hydrolases (UCHs), Ubiquitin-specific proteases (USPs) and deubiquitinating enzymes (DUBs). This protein is useful for inhibiting the hydrolysis of poly-Ubiquitin chains on substrate proteins in vitro, thus enhancing poly-Ubiquitin chain accumulation. Some DUBs, such as those of the JAMM class, are not inhibited by Ubiquitin-Vinyl Sulfone.

References
  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
  8. Borodovsky A., et al. (2001) EMBO J. 20: 5187-5196.
  9. Borodovsky A., et al. (2002) Chem. Biol. 9: 1149-1159.
  10. Borodovsky A., et al. (2005) Chem. Biochem. 6: 287-291.
  11. Hemelaar J., et al. (2004) Mol. Cell. Bio. 24: 84-95.
  12. Kessler B.M. (2006) Exp. Rev. Proteomics. 3 :213-221.
  13. Misaghi S., et al. (2005) J. Biol. Chem. 280: 1512-1520.
  14. Wang G., et al. (2003) Org. Lett. 5: 737-740.
  15. Verma R., et al. (2002) Science 298: 611-615.
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Citations for Synthetic Human Ubiquitin Vinyl Sulfone Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. USP14-regulated allostery of the human proteasome by time-resolved cryo-EM
    Authors: S Zhang, S Zou, D Yin, L Zhao, D Finley, Z Wu, Y Mao
    Nature, 2022-04-27;605(7910):567-574.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  2. A molecular sensor determines the ubiquitin substrate specificity of SARS-CoV-2 papain-like protease
    Authors: S Patchett, Z Lv, W Rut, M Békés, M Drag, SK Olsen, TT Huang
    Cell Reports, 2021-09-08;36(13):109754.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Two distinct modes of DNMT1 recruitment ensure stable maintenance DNA methylation
    Authors: A Nishiyama, CB Mulholland, S Bultmann, S Kori, A Endo, Y Saeki, W Qin, C Trummer, Y Chiba, H Yokoyama, S Kumamoto, T Kawakami, H Hojo, G Nagae, H Aburatani, K Tanaka, K Arita, H Leonhardt, M Nakanishi
    Nat Commun, 2020-03-06;11(1):1222.
    Species: Xenopus
    Sample Types: Egg Extract
    Applications: Bioassay
  4. Uncoupling of p97 ATPase activity has a dominant negative effect on protein extraction
    Authors: HB Rycenga, KB Wolfe, ES Yeh, DT Long
    Sci Rep, 2019-07-17;9(1):10329.
    Species: Xenopus
    Sample Types: Egg Extract
    Applications: Bioassay
  5. DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response
    Authors: F Liang, AS Miller, S Longerich, C Tang, D Maranon, EA Williamson, R Hromas, C Wiese, GM Kupfer, P Sung
    Nat Commun, 2019-06-28;10(1):2849.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. TRIM11 activates the proteasome and promotes overall protein degradation by regulating USP14
    Authors: L Chen, G Zhu, EM Johns, X Yang
    Nat Commun, 2018-03-26;9(1):1223.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  7. An inhibitor of the proteasomal deubiquitinating enzyme USP14 induces tau elimination in cultured neurons
    Authors: M Boselli, BH Lee, J Robert, MA Prado, SW Min, C Cheng, MC Silva, C Seong, S Elsasser, KM Hatle, TC Gahman, SP Gygi, SJ Haggarty, L Gan, RW King, D Finley
    J. Biol. Chem., 2017-09-26;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Enzyme Assay
  8. Usp7-dependent histone H3 deubiquitylation regulates maintenance of DNA methylation
    Authors: L Yamaguchi, A Nishiyama, T Misaki, Y Johmura, J Ueda, K Arita, K Nagao, C Obuse, M Nakanishi
    Sci Rep, 2017-03-03;7(1):55.
    Species: Xenopus
    Sample Types: Tissue Culture Supernates
    Applications: Bioassay
  9. Deubiquitination and Activation of AMPK by USP10
    Authors: M Deng, X Yang, B Qin, T Liu, H Zhang, W Guo, SB Lee, JJ Kim, J Yuan, H Pei, L Wang, Z Lou
    Mol. Cell, 2016-02-11;61(4):614-24.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent phosphorylation.
    Authors: Iguchi, Masahiro, Kujuro, Yuki, Okatsu, Kei, Koyano, Fumika, Kosako, Hidetaka, Kimura, Mayumi, Suzuki, Norihiro, Uchiyama, Shinichi, Tanaka, Keiji, Matsuda, Noriyuki
    J Biol Chem, 2013-06-10;288(30):22019-32.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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