Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

Catalog #: SPCKB-MP-004049 Datasheet / COA / SDS

Simple Plex Mouse/Rat Osteopontin/OPN assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-004049
SPCKA-MP-004049
SPCKC-MP-004049

Control Products Available

898393: Simple Plex Control for Mouse/Rat Osteopontin/OPN

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse/Rat Osteopontin/OPN Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Serum (500 nL), Rat Serum (500 nL), Mouse EDTA Plasma (500 nL), Rat EDTA Plasma (500 nL), Mouse Heparin Plasma (500 nL), Mouse Urine (25 nL), Rat Urine (500 nL), Mouse Cerebrospinal Fluid (500 nL)

Sensitivity

0.28 pg/mL

Assay Range

1.2 - 4,580 pg/mL (Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Heparin Plasma, Mouse Heparin Plasma, Mouse Urine, Rat Urine, Cerebrospinal Fluid, Mouse Cerebrospinal Fluid)

Specificity

Natural and recombinant mouse/rat Osteopontin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse and rat Osteopontin in serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Rat EDTA Plasma, Heparin Plasma, Mouse Urine, Rat Urine, Cerebrospinal Fluid

	Intra-Assay Precision	Inter-Assay Precision
Sample	1
n	16
Mean (pg/mL)	24.7
Standard Deviation	1.03
CV%	4.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Urine (n=4)	108	96-120

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat Osteopontin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Mouse/Rat OPN Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Osteopontin/OPN

Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.

Long Name:

Secreted Phosphoprotein 1 [BNSP]

Entrez Gene IDs:

6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)

Alternate Names:

BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin