Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge

Catalog #: SPCKB-MP-008843 Datasheet / COA / SDS

Simple Plex Mouse/Rat Chitinase 3-like 1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD10, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-008843
SPCKA-MP-008843
SPCKC-MP-008843

Control Products Available

897065: Simple Plex Control for Mouse/Rat Chitinase 3-like 1/YKL-40

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All Chitinase 3-like 1/YKL-40 Simple Plex Assays

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Summary Precision Recovery Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse/Rat Chitinase 3-like 1/YKL-40 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Bronchoalveolar Lavage (10 uL), Mouse Serum (10 uL), Rat Serum (10 uL), Mouse EDTA Plasma (10 uL), Rat EDTA Plasma (10 uL), Mouse Heparin Plasma (10 uL), Rat Heparin Plasma (10 uL), Mouse Cerebrospinal Fluid (10 uL)

Sensitivity

1.08 pg/mL

Assay Range

4.1 - 15,502 pg/mL (Mouse Bronchoalveolar Lavage, Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Cerebrospinal Fluid)

Specificity

Natural and recombinant mouse and rat CHI3L1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse and rat Chitinase 3-like 1 (CHI3L1) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin), bronchoalveolar lavage fluid (BALF), and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Bronchoalveolar Lavage, Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	41	43
Mean (pg/mL)	116	559	101	5135
Standard Deviation	3.64	439	10.8	469
CV%	3.1	7.8	10.7	9.1

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	116	115-117

Scientific Data

N/A

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Mouse/Rat CH3L1 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Chitinase 3-like 1/YKL-40

Chitinase 3-like 1 (CHI3L1), also called BRP39 in mouse or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.

Entrez Gene IDs:

1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)

Alternate Names:

AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40