Simple Plex Human Total Tau Cartridge

Catalog #: SPCKC-PS-009562 Datasheet / COA / SDS

Simple Plex Human Total Tau assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKC-PS-009562
SPCKA-PS-009562

Control Products Available

897083: Simple Plex Control for Human Total Tau

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All Tau Simple Plex Assays

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Summary Precision Recovery Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Total Tau Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Cerebrospinal Fluid (10 uL)

Sensitivity

0.13 pg/mL

Assay Range

0.8 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Specificity

Natural and recombinant Total Tau.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Total Tau in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	34	35
Mean (pg/mL)	70.7	2893	72.4	2818
Standard Deviation	5.55	260	5.7	204
CV%	7.9	9	7.9	7.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	104	99-108
EDTA Plasma (n=4)	105	102-109
Heparin Plasma (n=4)	104	101-108
Serum (n=4)	103	93-111

Scientific Data

N/A

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Human Total Tau Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Tau

Tau protein serves as a bridge from microtubules to the plasma membrane to facilitate formation and stabilization of the microtubule network. Tau in PNS neurons is 758 amino acids (aa) in length and 110 kDa in size, but multiple isoforms exist, ranging from 316 to 776 aa in length. Various deletions occur within the first 500 aa, with some isoforms also showing an absence of the second Tau repeat.

Long Name:

Microtubule-Associated Protein Tau

Entrez Gene IDs:

4137 (Human); 17762 (Mouse); 29477 (Rat)

Alternate Names:

DDPAC; FLJ31424; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPT; MGC138549; microtubule-associated protein tau; MSTD; MSTDMAPTL; MTBT1; MTBT1Neurofibrillary tangle protein; MTBT2; Neurofibrillary tangle protein; PHF-tau; PPND; Tau; TAUPaired helical filament-tau