Simple Plex Human S100B Cartridge

Catalog #: SPCKC-PS-009486 Datasheet / COA / SDS

Simple Plex Human S100B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD10, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKC-PS-009486
SPCKA-PS-009486
ST01C-PS-009486

Control Products Available

898575: Simple Plex Control for Human/Mouse/Rat S100B

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human S100B Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Cerebrospinal Fluid (10 uL)

Sensitivity

0.648 pg/mL

Assay Range

4.0 - 2,448 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Cerebrospinal Fluid)

Specificity

Natural and recombinant S100B.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human S100 Calcium Binding Protein B (S100B) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin), saliva, and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	72	71
Mean (pg/mL)	32	1845	23.5	1443
Standard Deviation	2.09	67.3	2.16	115
CV%	6.6	3.6	9.2	8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	101	93-108
EDTA Plasma (n=4)	96	92-99
Heparin Plasma (n=4)	94	90-98
Saliva (n=3)	89	82-94
Serum (n=4)	94	90-101

Linearity

Samples containing and/or spiked with high concentrations of S100B were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Human S100B Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: S100B

S100B belongs to the S100 subgroup of the EF-hand family of calcium binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.

Long Name:

S100 Calcium Binding Protein B

Entrez Gene IDs:

6285 (Human)

Alternate Names:

beta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100; S100B; S100beta