Simple Plex Human Neutrophil Elastase/ELA2 Cartridge New

Catalog #: SPCKB-PS-011934 Datasheet / COA / SDS

Simple Plex Human Neutrophil Elastase/ELA2 assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD06, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-011934
SPCKA-PS-011934
SPCKC-PS-011934

Control Products Available

897163: Simple Plex Control for Human Neutrophil Elastase/ELA2

Simple Plex Human Neutophil Elastase/ELA2 Ella Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Neutrophil Elastase/ELA2 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

3.97 pg/mL

Assay Range

16.4 - 10,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human ELA2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Human Neutrophil Elastase (ELA2) in cell culture supernates (CCS), serum, and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	78	53
Mean (pg/mL)	63.9	4161	68.5	3515
Standard Deviation	2.39	203	8.1	325
CV%	3.7	4.9	11.9	9.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	110	109-110

Linearity

Samples containing and/or spiked with high concentrations of human ELA2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Human Neutophil Elastase/ELA2 Ella Assay

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neutrophil Elastase/ELA2

Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function results in the degradation of many extracellular matrix macromolecules. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. ELA2 may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases.

Entrez Gene IDs:

1991 (Human); 50701 (Mouse)

Alternate Names:

Bone marrow serine protease; EC 3.4.21; EC 3.4.21.37; ELA2; ELA2granulocyte-derived elastase; ELANE; elastase 2, neutrophil; elastase, neutrophil expressed; Elastase-2; GE; HLEelastase-2; HNE; Human leukocyte elastase; Leukocyte Elastase; Medullasin; NE; Neutrophil Elastase; PMN elastase; PMN-E; polymorphonuclear elastase; SCN1