Simple Plex Human LAG-3 Cartridge

Catalog #: SPCKB-PS-001348 Datasheet / COA / SDS

Simple Plex Human LAG-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001348
SPCKA-PS-001348
SPCKC-PS-001348

Control Products Available

898736: Simple Plex Control for Human LAG-3

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human LAG-3 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

15 pg/mL

Assay Range

39.6 - 151,050 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human LAG-3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Lymphocyte Activation Gene 3 (LAG-3) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	61	60
Mean (pg/mL)	936	43772	972	45960
Standard Deviation	47.9	3730	81.5	4953
CV%	5.1	8.5	8.4	10.8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	102	101-102
EDTA Plasma (n=4)	105	97-110
Heparin Plasma (n=4)	102	92-114
Serum (n=4)	97	95-98

Linearity

Samples containing and/or spiked with high concentrations of human LAG-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human LAG-3 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LAG-3

LAG-3 (Lymphocyte activation gene-3; CD223) is a transmembrane protein that binds to MHC class II molecules and negatively regulates T cell receptor signaling. It is expressed on activated T cells, NK cells, and plasmacytoid dendritic cells (pDC). LAG-3 limits the expansion of activated T cells and pDC in response to select stimuli. Proteolytic shedding of LAG-3 enables normal T cell activation by removing the negative regulation. Binding of a homodimerized soluble LAG-3/Ig fusion protein to MHC class II molecules induces maturation of immature DC as well as secretion of pro-inflammatory cytokines by cytotoxic CD8+ T cells and NK cells.

Long Name:

Lymphocyte-activation Gene 3

Entrez Gene IDs:

3902 (Human); 16768 (Mouse); 297596 (Rat); 102122272 (Cynomolgus Monkey)

Alternate Names:

CD223 antigen; CD223; LAG3; LAG-3; lymphocyte activating 3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Secreted lymphocyte activation gene 3 protein; sLAG-3