Simple Plex Human IL-19 Cartridge

Catalog #: SPCKB-PS-002815 Datasheet / COA / SDS

Simple Plex Human IL-19 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-002815
SPCKA-PS-002815
SPCKC-PS-002815

Control Products Available

898983: Simple Plex Control for Human IL-19

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human IL-19 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Human Milk (10 uL)

Sensitivity

0.84 pg/mL

Assay Range

3.1 - 11,700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk)

Specificity

Natural and recombinant human IL-19.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IL-19 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and human milk.

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	96	83-108
Heparin Plasma (n=4)	96	88-106
Serum (n=4)	97	94-99

Linearity

Samples containing and/or spiked with high concentrations of human IL-19 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human IL-19 Simple Plex Assay Standard Curve

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Human IL-19 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-19

Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. IL-19 contains seven helices and is secreted as a 35 kDa glycosylated monomer. IL-19 expression has been observed in keratinocytes and monocytes. IL-19 binds a receptor complex consisting of IL-20R alpha and the IL-20 R beta. This receptor complex is also shared by IL-20 and IL-24.

Long Name:

Interleukin 19

Entrez Gene IDs:

29949 (Human); 329244 (Mouse)

Alternate Names:

IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19

Related Research Areas

FAQs

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