Simple Plex Human IL-1 beta Cartridge

Catalog #: SPCKB-PS-000216 Datasheet / COA / SDS
Simple Plex Human IL-1 beta /IL-1F2 assay kit contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum)
Catalog # Availability Size / Price Qty
SPCKB-PS-000216
SPCKA-PS-000216
SPCKC-PS-000216
ST01B-PS-000216
Control Products Available
Human IL-1 beta  Simpe Plex Assay
3 Images
Product Details
FAQs
Supplemental Products
Reviews

Simple Plex Human IL-1 beta Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
0.064 pg/mL
Assay Range
0.4 - 1,530 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-1 beta.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IL-1 beta in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin) on Ella automated benchtop ELISA. 

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 7 6
Mean (pg/mL) 8.66 446 8.42 410
Standard Deviation 0.427 16.5 0.342 23.3
CV% 4.9 3.7 4 5.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 106 97-120
Heparin Plasma (n=4) 99 92-112
Serum (n=4) 105 96-112

Linearity

Samples containing and/or spiked with high concentrations of human IL-1 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human IL-1 beta  Ella Assay Linearity

Scientific Data

Human IL-1 beta Ella Assay Standard Curve

Product Datasheets

You must select a language.

x

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.

Long Name:
Interleukin 1 beta
Entrez Gene IDs:
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

FAQs

No product specific FAQs exist for this product, however you may

View all Simple Plex Assays FAQs
Loading...

Reviews for Simple Plex Human IL-1 beta Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human IL-1 beta Cartridge and earn rewards!

Have you used Simple Plex Human IL-1 beta Cartridge?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review